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<p>Next-generation sequencing (NGS) technologies have achieved remarkable success in both biological research and clinical applications. However, in recent years, performance improvements have slowed due to fundamental limitations imposed by Poiseuille fluid dynamics in flow cells, which we overcome using Couette flow. Here we show NGS by roll-to-roll fluidics (r2r-fl), a cost-effective approach compatible with flexible biochip sizes. r2r-fl is a practical implementation of plane Couette flow, with up to 85-fold lower reagent consumption (US$0.16 per gigabase pair), rinsing times under 2 s and a reduction in paired-end 100-base pair sequencing turnaround from days to less than 12 h. The method maintains over 99.9% precision and 99.3% sensitivity of single nucleotide polymorphisms in the human genome, 99.9% mapping rate for <i>Escherichia coli</i>, and minimal nucleotide substitutions, deletions or insertions in the severe acute respiratory syndrome coronavirus 2 alpha strain. By lowering cost and time, r2r-fl enables rapid, scalable NGS for pathogen detection, cancer diagnostics and genetic disease profiling.</p>

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Fast, cost-effective and flexible DNA sequencing by roll-to-roll fluidics

  • 期刊论文

  • 发表日期：

    2025年7月14日

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  • Yanzhe Qin,
  • Stephan A. Koehler,
  • Yunyan Ling,
  • Shiqiang Yu,
  • Yongjie Zhang,
  • Jie Luo,
  • Kaijian Chen,
  • Luo Junjie,
  • Junjie Zeng,
  • Haiyang Chu,
  • Fei Wang,
  • Wei Li,
  • Dan Li,
  • Xinran Yu,
  • Xiangchao Wu,
  • Shengming Zhao,
  • Hao Lu,
  • Ziqing Deng,
  • Zhijian Yang,
  • Ruibin Mai,
  • Zhuo Liu,
  • Zihua Niu,
  • Xin Huang,
  • Chengmei Xing,
  • Xingcai Zhang,
  • Yongyou Hu,
  • Xinwen Peng,
  • Xiaoxing Liao,
  • Xiangmeng Qu,
  • Yiming Gong,
  • Qiushui Chen,
  • Thomas M. Hermans,
  • Wenwei Zhang,
  • Huanghao Yang

摘要

Next-generation sequencing (NGS) technologies have achieved remarkable success in both biological research and clinical applications. However, in recent years, performance improvements have slowed due to fundamental limitations imposed by Poiseuille fluid dynamics in flow cells, which we overcome using Couette flow. Here we show NGS by roll-to-roll fluidics (r2r-fl), a cost-effective approach compatible with flexible biochip sizes. r2r-fl is a practical implementation of plane Couette flow, with up to 85-fold lower reagent consumption (US$0.16 per gigabase pair), rinsing times under 2 s and a reduction in paired-end 100-base pair sequencing turnaround from days to less than 12 h. The method maintains over 99.9% precision and 99.3% sensitivity of single nucleotide polymorphisms in the human genome, 99.9% mapping rate for Escherichia coli, and minimal nucleotide substitutions, deletions or insertions in the severe acute respiratory syndrome coronavirus 2 alpha strain. By lowering cost and time, r2r-fl enables rapid, scalable NGS for pathogen detection, cancer diagnostics and genetic disease profiling.