Scholarlink

<p>Plant cells are contained within a rigid network of cell walls. Cell walls serve as a structural material and a crucial signaling hub vital to all aspects of the plant life cycle. However, many features of the cell wall remain enigmatic, as it has been challenging to map its functional properties in live plants at subcellular resolution. Here, we introduce CarboTag, a modular toolbox for live functional imaging of plant walls. CarboTag uses a small molecular motif, a pyridine boronic acid, that directs its cargo to the cell wall. We designed a suite of cell wall imaging probes based on CarboTag in various colors for multiplexing. Additionally, we developed new functional reporters for live quantitative imaging of key cell wall characteristics: network porosity, cell wall pH and the presence of reactive oxygen species. CarboTag paves the way for dynamic and quantitative mapping of cell wall responses at subcellular resolution.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

CarboTag: a modular approach for live and functional imaging of plant cell walls

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年5月1日

查看全文
  • Maarten Besten,
  • Milan Hendriksz,
  • Lucile Michels,
  • Bénédicte Charrier,
  • Elwira Smakowska-Luzan,
  • Dolf Weijers,
  • Jan Willem Borst,
  • Joris Sprakel

摘要

Plant cells are contained within a rigid network of cell walls. Cell walls serve as a structural material and a crucial signaling hub vital to all aspects of the plant life cycle. However, many features of the cell wall remain enigmatic, as it has been challenging to map its functional properties in live plants at subcellular resolution. Here, we introduce CarboTag, a modular toolbox for live functional imaging of plant walls. CarboTag uses a small molecular motif, a pyridine boronic acid, that directs its cargo to the cell wall. We designed a suite of cell wall imaging probes based on CarboTag in various colors for multiplexing. Additionally, we developed new functional reporters for live quantitative imaging of key cell wall characteristics: network porosity, cell wall pH and the presence of reactive oxygen species. CarboTag paves the way for dynamic and quantitative mapping of cell wall responses at subcellular resolution.