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Microsecond time-resolved X-ray scattering by utilizing MHz repetition rate at second-generation XFELs

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2024年7月5日

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  • Patrick E. Konold,
  • Leonardo Monrroy,
  • Alfredo Bellisario,
  • Diogo Filipe,
  • Patrick Adams,
  • Roberto Alvarez,
  • Richard Bean,
  • Johan Bielecki,
  • Szabolcs Bódizs,
  • Gabriel Ducrocq,
  • Helmut Grubmueller,
  • Richard A. Kirian,
  • Marco Kloos,
  • Jayanath C. P. Koliyadu,
  • Faisal H. M. Koua,
  • Taru Larkiala,
  • Romain Letrun,
  • Fredrik Lindsten,
  • Michael Maihöfer,
  • Andrew V. Martin,
  • Petra Mészáros,
  • Jennifer Mutisya,
  • Amke Nimmrich,
  • Kenta Okamoto,
  • Adam Round,
  • Tokushi Sato,
  • Joana Valerio,
  • Daniel Westphal,
  • August Wollter,
  • Tej Varma Yenupuri,
  • Tong You,
  • Filipe Maia,
  • Sebastian Westenhoff

摘要

Detecting microsecond structural perturbations in biomolecules has wide relevance in biology, chemistry and medicine. Here we show how MHz repetition rates at X-ray free-electron lasers can be used to produce microsecond time-series of protein scattering with exceptionally low noise levels of 0.001%. We demonstrate the approach by examining Jɑ helix unfolding of a light-oxygen-voltage photosensory domain. This time-resolved acquisition strategy is easy to implement and widely applicable for direct observation of structural dynamics of many biochemical processes.