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<p>Chalkiness and seed vigor are major agronomic traits affecting rice grain quality and productivity, respectively. Little is known about the intrinsic relationship and the regulatory mechanisms of these two traits. Here we identified a chalkiness locus <i>qPGWC-8</i>, which encodes trehalose-6-phosphate synthase 8 (OsTPS8) that suppresses trehalose-6-phosphate (Tre6P) biosynthesis by interacting with and inhibiting OsTPS1. A natural promoter variation in <i>OsTPS8</i> confers differential transcriptional regulation by OsbHLH001. Elevated expression of <i>OsTPS8</i> reduces Tre6P levels and activates the expression of <i>OsMYBS1</i> and α-amylase genes, thus promoting starch degradation, chalkiness formation and seed vigor elevation. Two major haplotypes, <i>OsTPS8</i><sup><i>ASO</i></sup> and <i>OsTPS8</i><sup><i>IR24</i></sup>, were defined by this functional promoter variation, with <i>OsTPS8</i><sup><i>IR24</i></sup> preferentially selected during the domestication of <i>indica</i> rice. Collectively, our findings establish an OsbHLH001-OsTPS8-Tre6P-α-amylase signaling cascade that has a dual role in regulating grain chalkiness and seed vigor, which reveals a molecular link between the appearance quality and seed vigor of rice.</p>

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Natural variation in OsTPS8 confers differential regulation of chalkiness and seed vigor in indica and japonica rice

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年12月15日

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  • Xiaoli Chen,
  • Yulong Ren,
  • Hui Dong,
  • Xiaokang Jiang,
  • Xiaoming Zheng,
  • Erchao Duan,
  • Xuan Teng,
  • Yunlong Wang,
  • Chuanwei Gu,
  • Rongbo Chen,
  • Qingkai Wang,
  • Yongfei Wang,
  • Yipeng Zhang,
  • Rushuang Zhang,
  • Yunpeng Zhang,
  • Wenjie Zhao,
  • Yu Zhang,
  • Xue Yang,
  • Lei Zhou,
  • Chao Li,
  • Tiaofeng Shan,
  • Yiqun Bao,
  • Yunlu Tian,
  • Xi Liu,
  • Shijia Liu,
  • Tao Guo,
  • Mingjiang Chen,
  • Haiyang Wang,
  • Yihua Wang,
  • Jianmin Wan

摘要

Chalkiness and seed vigor are major agronomic traits affecting rice grain quality and productivity, respectively. Little is known about the intrinsic relationship and the regulatory mechanisms of these two traits. Here we identified a chalkiness locus qPGWC-8, which encodes trehalose-6-phosphate synthase 8 (OsTPS8) that suppresses trehalose-6-phosphate (Tre6P) biosynthesis by interacting with and inhibiting OsTPS1. A natural promoter variation in OsTPS8 confers differential transcriptional regulation by OsbHLH001. Elevated expression of OsTPS8 reduces Tre6P levels and activates the expression of OsMYBS1 and α-amylase genes, thus promoting starch degradation, chalkiness formation and seed vigor elevation. Two major haplotypes, OsTPS8ASO and OsTPS8IR24, were defined by this functional promoter variation, with OsTPS8IR24 preferentially selected during the domestication of indica rice. Collectively, our findings establish an OsbHLH001-OsTPS8-Tre6P-α-amylase signaling cascade that has a dual role in regulating grain chalkiness and seed vigor, which reveals a molecular link between the appearance quality and seed vigor of rice.