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<p>Wheat stripe rust poses a major threat to global food security. Discovery of disease resistance genes from wild relatives enables multigene stacking that could enhance durability. Here we use map-based cloning and long-read sequencing to isolate two adjacent nucleotide-binding and leucine-rich repeat (NLR) receptors from wild emmer wheat. Using mutagenesis, gene silencing and genetic transformation, we show that the genes <i>TdNLR1</i> and <i>TdNLR2</i> oriented head-to-head are both required for <i>YrTD121-</i>mediated stripe rust resistance. <i>TdNLR1</i> encodes a canonical NLR (CC-NB-ARC-LRR) protein, whereas <i>TdNLR2</i> encodes an atypical one (NB-ARC-LRR). Both genes lack an integrated domain previously associated with effector perception, representing an uncommon architecture for paired NLRs in plants. The coiled coil domain of <i>TdNLR1</i> triggers cell death and self-associates in planta. <i>YrTD121</i> was present in wild emmer but absent in all other <i>Triticum</i> species examined. Our work sheds light on the function of paired NLRs in conferring disease resistance and facilitates breeding for resistance.</p>

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A head-to-head NLR gene pair from wild emmer confers stripe rust resistance in wheat

  • 期刊论文

  • 发表日期：

    2025年6月9日

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  • Yanling Hu,
  • Miaomiao Li,
  • Yuqin Li,
  • Lilin Du,
  • Ruijie Xie,
  • Fei Ni,
  • Chongjing Xia,
  • Ke Wang,
  • Yanyan Huang,
  • Binyang Xu,
  • Yinghui Li,
  • Yunfeng Jiang,
  • Ming Hao,
  • Bo Jiang,
  • Shunzong Ning,
  • Zhongwei Yuan,
  • Lihua Feng,
  • Lianquan Zhang,
  • Shisheng Chen,
  • Bihua Wu,
  • Zhiyong Liu,
  • Tzion Fahima,
  • Dengcai Liu,
  • Lin Huang

摘要

Wheat stripe rust poses a major threat to global food security. Discovery of disease resistance genes from wild relatives enables multigene stacking that could enhance durability. Here we use map-based cloning and long-read sequencing to isolate two adjacent nucleotide-binding and leucine-rich repeat (NLR) receptors from wild emmer wheat. Using mutagenesis, gene silencing and genetic transformation, we show that the genes TdNLR1 and TdNLR2 oriented head-to-head are both required for YrTD121-mediated stripe rust resistance. TdNLR1 encodes a canonical NLR (CC-NB-ARC-LRR) protein, whereas TdNLR2 encodes an atypical one (NB-ARC-LRR). Both genes lack an integrated domain previously associated with effector perception, representing an uncommon architecture for paired NLRs in plants. The coiled coil domain of TdNLR1 triggers cell death and self-associates in planta. YrTD121 was present in wild emmer but absent in all other Triticum species examined. Our work sheds light on the function of paired NLRs in conferring disease resistance and facilitates breeding for resistance.