Scholarlink

<p>Cotton (<i>Gossypium hirsutum</i> L.) is a key allopolyploid crop with global economic importance. Here we present a telomere-to-telomere assembly of the elite variety Zhongmian 113. Leveraging technologies including PacBio HiFi, Oxford Nanopore Technology (ONT) ultralong-read sequencing and Hi-C, our assembly surpasses previous genomes in contiguity and completeness, resolving 26 centromeric and 52 telomeric regions, 5S rDNA clusters and nucleolar organizer regions. A phylogenetically recent centromere repositioning on chromosome D08 was discovered specific to <i>G. hirsutum</i>, involving deactivation of an ancestral centromere and the formation of a unique, satellite repeat-based centromere. Genomic analyses evaluated favorable allele aggregation for key agronomic traits and uncovered an early-maturing haplotype derived from an 11 Mb pericentric inversion that evolved early during <i>G. hirsutum</i> domestication. Our study sheds light on the genomic origins of short-season adaptation, potentially involving introgression of an inversion from primitively domesticated forms, followed by subsequent haplotype differentiation in modern breeding programs.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A telomere-to-telomere genome assembly of cotton provides insights into centromere evolution and short-season adaptation

  • 期刊论文

  • 发表日期：

    2025年3月17日

查看全文
  • Guanjing Hu,
  • Zhenyu Wang,
  • Zunzhe Tian,
  • Kai Wang,
  • Gaoxiang Ji,
  • Xingxing Wang,
  • Xianliang Zhang,
  • Zhaoen Yang,
  • Xuan Liu,
  • Ruoyu Niu,
  • De Zhu,
  • Yuzhi Zhang,
  • Lian Duan,
  • Xueyuan Ma,
  • Xianpeng Xiong,
  • Jiali Kong,
  • Xianjia Zhao,
  • Ya Zhang,
  • Junjie Zhao,
  • Shoupu He,
  • Corrinne E. Grover,
  • Junji Su,
  • Keyun Feng,
  • Guangrun Yu,
  • Jinlei Han,
  • Xinshan Zang,
  • Zhiqiang Wu,
  • Weihua Pan,
  • Jonathan F. Wendel,
  • Xiongfeng Ma

摘要

Cotton (Gossypium hirsutum L.) is a key allopolyploid crop with global economic importance. Here we present a telomere-to-telomere assembly of the elite variety Zhongmian 113. Leveraging technologies including PacBio HiFi, Oxford Nanopore Technology (ONT) ultralong-read sequencing and Hi-C, our assembly surpasses previous genomes in contiguity and completeness, resolving 26 centromeric and 52 telomeric regions, 5S rDNA clusters and nucleolar organizer regions. A phylogenetically recent centromere repositioning on chromosome D08 was discovered specific to G. hirsutum, involving deactivation of an ancestral centromere and the formation of a unique, satellite repeat-based centromere. Genomic analyses evaluated favorable allele aggregation for key agronomic traits and uncovered an early-maturing haplotype derived from an 11 Mb pericentric inversion that evolved early during G. hirsutum domestication. Our study sheds light on the genomic origins of short-season adaptation, potentially involving introgression of an inversion from primitively domesticated forms, followed by subsequent haplotype differentiation in modern breeding programs.