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<p>Current high-throughput DNA synthesis technologies use intricate chip and microfluidic systems to produce large-scale synthetic oligonucleotides but with low concentration and limited compatibility for long DNA assembly. Here we report a massive-in-parallel synthesis system, with an ‘identification–sorting–synthesis–recycling’ iteration mechanism applied to microchips for high-throughput DNA synthesis. This approach increases DNA product concentration by four to six orders of magnitude and simplifies downstream processes for large-scale gene synthesis.</p>

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Scaling DNA synthesis with a microchip-based massively parallel synthesis system

  • 期刊论文

  • 发表日期：

    2025年10月1日

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  • Xiandi Zhang,
  • Xiang’er Jiang,
  • Yun Wang,
  • Qinzhen Chen,
  • Hao Jiang,
  • Hu Zhang,
  • Antoni Beltran,
  • Weiya Yang,
  • Tai Chen,
  • Chenglong Liang,
  • Ning Cheng,
  • Yun Huang,
  • Guqiao Ding,
  • Chengwang Xie,
  • Nanfeng Gao,
  • Juntao Liu,
  • Wei Xu,
  • Jinlei Huang,
  • Dong Cai,
  • Lihao Zhu,
  • Songjin Mo,
  • Mengzhe Shen,
  • Wenwei Zhang,
  • Ben Lehner,
  • Ming Ni,
  • Jian Wang,
  • Xun Xu,
  • Yue Shen

摘要

Current high-throughput DNA synthesis technologies use intricate chip and microfluidic systems to produce large-scale synthetic oligonucleotides but with low concentration and limited compatibility for long DNA assembly. Here we report a massive-in-parallel synthesis system, with an ‘identification–sorting–synthesis–recycling’ iteration mechanism applied to microchips for high-throughput DNA synthesis. This approach increases DNA product concentration by four to six orders of magnitude and simplifies downstream processes for large-scale gene synthesis.