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<p>We perform intact proteoform profiling of 10,809 endogenous single cells from the rat hippocampus using single-cell proteoform imaging mass spectrometry (scPiMS). scPiMS directly extracts whole proteins and demonstrates high throughput for MS-based single-cell proteomics compared with existing approaches. We develop an informatics workflow dedicated to this datatype and use it to assign neurons, astrocytes or microglia cell types according to their proteoform signatures.</p>

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Proteoform profiling of endogenous single cells from rat hippocampus at scale

  • 期刊论文

  • 发表日期：

    2025年5月15日

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  • Pei Su,
  • Michael A. R. Hollas,
  • Indira Pla,
  • Stanislav Rubakhin,
  • Fatma Ayaloglu Butun,
  • Joseph B. Greer,
  • Bryan P. Early,
  • Ryan T. Fellers,
  • Michael A. Caldwell,
  • Jonathan V. Sweedler,
  • Jared O. Kafader,
  • Neil L. Kelleher

摘要

We perform intact proteoform profiling of 10,809 endogenous single cells from the rat hippocampus using single-cell proteoform imaging mass spectrometry (scPiMS). scPiMS directly extracts whole proteins and demonstrates high throughput for MS-based single-cell proteomics compared with existing approaches. We develop an informatics workflow dedicated to this datatype and use it to assign neurons, astrocytes or microglia cell types according to their proteoform signatures.