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Intravenous administration of blood–brain barrier-crossing conjugates facilitate biomacromolecule transport into central nervous system

  • Chang Wang,
  • Siyu Wang,
  • Yonger Xue,
  • Yichen Zhong,
  • Haoyuan Li,
  • Xucheng Hou,
  • Diana D. Kang,
  • Zhengwei Liu,
  • Meng Tian,
  • Leiming Wang,
  • Dinglingge Cao,
  • Yang Yu,
  • Jayce Liu,
  • Xiaolin Cheng,
  • Tamara Markovic,
  • Alice Hashemi,
  • Brian H. Kopell,
  • Alexander W. Charney,
  • Eric J. Nestler,
  • Yizhou Dong

摘要

Delivery of biomacromolecules to the central nervous system (CNS) remains challenging because of the restrictive nature of the blood–brain barrier (BBB). We developed a BBB-crossing conjugate (BCC) system that facilitates delivery into the CNS through γ-secretase-mediated transcytosis. Intravenous administration of a BCC10–oligonucleotide conjugate demonstrated effective transportation of the oligonucleotide across the BBB and gene silencing in wild-type mice, human brain tissues and an amyotrophic lateral sclerosis mouse model.