Scholarlink

<p>As the fundamental executors of biological function, proteins are frequently dysregulated or differentially expressed in disease states, making them valuable biomarkers and/or therapeutic targets. Conventional approaches to monitoring the presence and activity of these proteins — including enzyme-linked immunosorbent assay, western blotting and mass spectrometry — have limited ability to provide real-time information on living cells. Fluorescence imaging overcomes these limitations by enabling selective, non-invasive and dynamic protein analysis. Molecular rotor fluorophores offer unique advantages owing to their high sensitivity to microenvironmental changes and tunable photophysical properties. Notably, these molecular rotor scaffolds can be functionalized into targeting probes that become highly emissive upon binding to specific proteins via the restriction of intramolecular rotation. Here, we introduce molecular rotor-based probes and outline their design principles and detection mechanisms. We highlight their applications in disease diagnosis and biological research, and we discuss the current challenges and prospects for clinical translation.</p><p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Molecular rotor-based probes for protein monitoring in biomedical research

  • 期刊论文

  • 发表日期：

    2026年4月29日

查看全文
  • Xia Wu,
  • Yuxuan Hu,
  • Quan Wang,
  • Wei Zhang,
  • Tao Liu,
  • Fan Xia,
  • Xiaoding Lou,
  • Kanyi Pu

摘要

As the fundamental executors of biological function, proteins are frequently dysregulated or differentially expressed in disease states, making them valuable biomarkers and/or therapeutic targets. Conventional approaches to monitoring the presence and activity of these proteins — including enzyme-linked immunosorbent assay, western blotting and mass spectrometry — have limited ability to provide real-time information on living cells. Fluorescence imaging overcomes these limitations by enabling selective, non-invasive and dynamic protein analysis. Molecular rotor fluorophores offer unique advantages owing to their high sensitivity to microenvironmental changes and tunable photophysical properties. Notably, these molecular rotor scaffolds can be functionalized into targeting probes that become highly emissive upon binding to specific proteins via the restriction of intramolecular rotation. Here, we introduce molecular rotor-based probes and outline their design principles and detection mechanisms. We highlight their applications in disease diagnosis and biological research, and we discuss the current challenges and prospects for clinical translation.