<p>DNA nanotechnology has rapidly evolved, leading to the development of dynamic nanoscale and microscale devices that mimic natural molecular machinery. This Review explores the latest advancements in DNA-based machines, motors and switches, emphasizing the need for clear definitions to distinguish between these often-interchanged terms. By analysing key performance metrics such as speed, force generation, efficiency and autonomy, we provide a framework for evaluating these devices against their biological counterparts, including motor proteins such as myosin and kinesin. We highlight innovative design strategies such as strand displacement, DNA origami and hybrid systems, which enhance the functionality of DNA-based constructs and bridge the gap between synthetic and natural systems. These advancements have promising applications in areas such as targeted drug delivery, biosensing and nanofabrication, although challenges in achieving the high performance and efficiency seen in biological systems remain. Through a synthesis of current research, this Review outlines the opportunities and challenges in the development of DNA-based nanoscale and microscale devices.</p><p></p>