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<p>Brillouin light scattering (BLS) spectroscopy is a non-invasive, non-contact, label-free optical technique that can provide information on the mechanical properties of a material on the submicrometre scale. Over the past decade, BLS has found increasing microscopy applications in the life sciences, driven by the observed importance of mechanical properties in biological processes, the realization of more sensitive BLS spectrometers and the extension of BLS to an imaging modality. As with other spectroscopic techniques, BLS measurements detect not only signals that are characteristic of the investigated sample, but also those of the experimental apparatus, and can be substantially affected by measurement conditions. Here we report a consensus between researchers in the field. We aim to improve the comparability of BLS studies by providing reporting recommendations for the measured parameters and detailing common artefacts. Given that most BLS studies of biological matter are still at proof-of-concept stages and use different, often self-built, spectrometers, a consensus statement is particularly timely to ensure unified advancement.</p>

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Consensus statement on Brillouin light scattering microscopy of biological materials

  • 期刊论文

  • 发表日期：

    2025年7月3日

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  • Pierre Bouvet,
  • Carlo Bevilacqua,
  • Yogeshwari Ambekar,
  • Giuseppe Antonacci,
  • Joshua Au,
  • Silvia Caponi,
  • Sophie Chagnon-Lessard,
  • Juergen Czarske,
  • Thomas Dehoux,
  • Daniele Fioretto,
  • Yujian Fu,
  • Jochen Guck,
  • Thorsten Hamann,
  • Dag Heinemann,
  • Torsten Jähnke,
  • Hubert Jean-Ruel,
  • Irina Kabakova,
  • Kristie Koski,
  • Nektarios Koukourakis,
  • David Krause,
  • Salvatore La Cavera III,
  • Timm Landes,
  • Jinhao Li,
  • Hadi Mahmodi,
  • Jeremie Margueritat,
  • Maurizio Mattarelli,
  • Michael Monaghan,
  • Darryl R. Overby,
  • Fernando Perez-Cota,
  • Emanuele Pontecorvo,
  • Robert Prevedel,
  • Giancarlo Ruocco,
  • John Sandercock,
  • Giuliano Scarcelli,
  • Filippo Scarponi,
  • Claudia Testi,
  • Peter Török,
  • Lucie Vovard,
  • Wolfgang J. Weninger,
  • Vladislav Yakovlev,
  • Seok-Hyun Yun,
  • Jitao Zhang,
  • Francesca Palombo,
  • Alberto Bilenca,
  • Kareem Elsayad

摘要

Brillouin light scattering (BLS) spectroscopy is a non-invasive, non-contact, label-free optical technique that can provide information on the mechanical properties of a material on the submicrometre scale. Over the past decade, BLS has found increasing microscopy applications in the life sciences, driven by the observed importance of mechanical properties in biological processes, the realization of more sensitive BLS spectrometers and the extension of BLS to an imaging modality. As with other spectroscopic techniques, BLS measurements detect not only signals that are characteristic of the investigated sample, but also those of the experimental apparatus, and can be substantially affected by measurement conditions. Here we report a consensus between researchers in the field. We aim to improve the comparability of BLS studies by providing reporting recommendations for the measured parameters and detailing common artefacts. Given that most BLS studies of biological matter are still at proof-of-concept stages and use different, often self-built, spectrometers, a consensus statement is particularly timely to ensure unified advancement.