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<p>The impact of nanomaterial transformations on photosynthetic proteins remains largely unknown. We report positively charged iron oxide (Fe<sub>3</sub>O<sub>4</sub>) nanoparticles experience transformations in <i>Arabidopsis thaliana</i> plants in vivo that alter the formation and function of RuBisCO protein corona, a key carbon fixation enzyme. In vitro, negatively charged Fe<sub>3</sub>O<sub>4</sub> nanoparticles impact the RuBisCO function but not their positively charged counterparts. Computational and in vitro proteomic analyses revealed that positively charged Fe<sub>3</sub>O<sub>4</sub> nanoparticles preferentially bind to a RuBisCO small subunit that lacks active carboxylation sites. However, both positively and negatively charged nanoparticles decrease RuBisCO carboxylation activity after experiencing transformations in vivo by 3.0 and 1.7 times relative to the controls, respectively. The pH- and lipid-coating-dependent transformations that occur during nanoparticle transport across plant membranes enhance RuBisCO binding to positively charged nanoparticles affecting its distribution in chloroplasts. Elucidating the rules of how nanoparticle properties and transformations affect photosynthetic coronas is crucial for sustainable nano-enabled agriculture.</p>

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In vivo transformations of positively charged nanoparticles alter the formation and function of RuBisCO photosynthetic protein corona

  • 期刊论文

  • 发表日期：

    2025年6月3日

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  • Christopher Castillo,
  • Su-Ji Jeon,
  • Khoi Nguyen L. Hoang,
  • Claire Alford,
  • Erica Svendahl,
  • Chaoyi Deng,
  • Yi Wang,
  • Yinhan Wang,
  • Xingfei Wei,
  • Rigoberto Hernandez,
  • Jason C. White,
  • Korin E. Wheeler,
  • Catherine J. Murphy,
  • Juan Pablo Giraldo

摘要

The impact of nanomaterial transformations on photosynthetic proteins remains largely unknown. We report positively charged iron oxide (Fe3O4) nanoparticles experience transformations in Arabidopsis thaliana plants in vivo that alter the formation and function of RuBisCO protein corona, a key carbon fixation enzyme. In vitro, negatively charged Fe3O4 nanoparticles impact the RuBisCO function but not their positively charged counterparts. Computational and in vitro proteomic analyses revealed that positively charged Fe3O4 nanoparticles preferentially bind to a RuBisCO small subunit that lacks active carboxylation sites. However, both positively and negatively charged nanoparticles decrease RuBisCO carboxylation activity after experiencing transformations in vivo by 3.0 and 1.7 times relative to the controls, respectively. The pH- and lipid-coating-dependent transformations that occur during nanoparticle transport across plant membranes enhance RuBisCO binding to positively charged nanoparticles affecting its distribution in chloroplasts. Elucidating the rules of how nanoparticle properties and transformations affect photosynthetic coronas is crucial for sustainable nano-enabled agriculture.