Scholarlink

<p>Animal–microorganism symbioses are omnipresent, with both partners often gaining benefits as mutualists. A single mutation in the carbon catabolite repression system in <i>Escherichia coli</i> enables mutualism with the stinkbug <i>Plautia stali</i>. Here we find that this mutation is not present in natural symbioses. Given that the carbon catabolite repression pathway affects the expression of &gt;500 downstream genes, we investigated their role in mutualisms. We find that disruption of a single gene, <i>tnaA</i>, encoding tryptophanase makes <i>E. coli</i> mutualistic to <i>P. stali</i>, resulting in the accumulation of tryptophan and the reduction of toxic indole. A survey of wild populations of <i>P. stali</i> and other stinkbug species revealed that their typical microbial symbionts, <i>Pantoea</i>, consistently lack the <i>tnaA</i> gene. Some <i>Pantoea</i> species such as <i>Pantoea ananatis</i> retain the <i>tnaA</i> gene and cannot establish symbiosis with <i>P. stali</i>, but <i>tnaA</i>-disrupted <i>P. ananatis</i> partially restored the symbiotic capability. When a natural <i>Pantoea</i> mutualist of <i>P. stali</i> was transformed with a functional <i>tna</i> operon, its symbiotic capability reduced significantly. Our finding suggests that tryptophanase disruption may have facilitated the evolution of gut bacterial mutualists in insects.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Tryptophanase disruption promotes insect–bacterium mutualism

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年2月27日

查看全文
  • Yayun Wang,
  • Minoru Moriyama,
  • Ryuichi Koga,
  • Kohei Oguchi,
  • Takahiro Hosokawa,
  • Hiroki Takai,
  • Shuji Shigenobu,
  • Naruo Nikoh,
  • Takema Fukatsu

摘要

Animal–microorganism symbioses are omnipresent, with both partners often gaining benefits as mutualists. A single mutation in the carbon catabolite repression system in Escherichia coli enables mutualism with the stinkbug Plautia stali. Here we find that this mutation is not present in natural symbioses. Given that the carbon catabolite repression pathway affects the expression of >500 downstream genes, we investigated their role in mutualisms. We find that disruption of a single gene, tnaA, encoding tryptophanase makes E. coli mutualistic to P. stali, resulting in the accumulation of tryptophan and the reduction of toxic indole. A survey of wild populations of P. stali and other stinkbug species revealed that their typical microbial symbionts, Pantoea, consistently lack the tnaA gene. Some Pantoea species such as Pantoea ananatis retain the tnaA gene and cannot establish symbiosis with P. stali, but tnaA-disrupted P. ananatis partially restored the symbiotic capability. When a natural Pantoea mutualist of P. stali was transformed with a functional tna operon, its symbiotic capability reduced significantly. Our finding suggests that tryptophanase disruption may have facilitated the evolution of gut bacterial mutualists in insects.