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<p>Phenazines are highly prevalent, natural bioactive substances secreted by microbes. However, their mode of action and potential involvement in shaping microbiomes remain elusive. Here we performed a comprehensive analysis of over 1.35 million bacterial genomes to identify phenazine-producing bacteria distributed across 193 species in 34 families. Analysis of rhizosphere microbiome and public rhizosphere metagenomic datasets revealed that phenazines could shape the microbial community by inhibiting Gram-positive bacteria, which was verified by pairwise interaction assays using Phenazine-1-carboxamide (PCN)-producing <i>Pseudomonas chlororaphis</i>. PCN induced DNA damage in <i>Bacillus subtilis</i>, a model Gram-positive target, where it directly bound to the bacterial topoisomerase IV, inhibiting its decatenation activity and leading to cell death. A two-species consortium of phenazine-producing <i>Pseudomonas</i> and resistant <i>B. subtilis</i> exhibited superior synergistic activity in preventing Fusarium crown rot in wheat plants. This work advances our understanding of a prevalent microbial interaction and its potential for biocontrol.</p>

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Phenazines contribute to microbiome dynamics by targeting topoisomerase IV

  • 期刊论文

  • 发表日期：

    2025年9月11日

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  • Yaqi Zhou,
  • Hongkai Wang,
  • Jiaxin Sun,
  • Wisnu Adi Wicaksono,
  • Chao Liu,
  • Yinghao He,
  • Yuxuan Qin,
  • Gabriele Berg,
  • Lei Li,
  • Houwen Lin,
  • Yunrong Chai,
  • Yang Bai,
  • Zhonghua Ma,
  • Tomislav Cernava,
  • Yun Chen

摘要

Phenazines are highly prevalent, natural bioactive substances secreted by microbes. However, their mode of action and potential involvement in shaping microbiomes remain elusive. Here we performed a comprehensive analysis of over 1.35 million bacterial genomes to identify phenazine-producing bacteria distributed across 193 species in 34 families. Analysis of rhizosphere microbiome and public rhizosphere metagenomic datasets revealed that phenazines could shape the microbial community by inhibiting Gram-positive bacteria, which was verified by pairwise interaction assays using Phenazine-1-carboxamide (PCN)-producing Pseudomonas chlororaphis. PCN induced DNA damage in Bacillus subtilis, a model Gram-positive target, where it directly bound to the bacterial topoisomerase IV, inhibiting its decatenation activity and leading to cell death. A two-species consortium of phenazine-producing Pseudomonas and resistant B. subtilis exhibited superior synergistic activity in preventing Fusarium crown rot in wheat plants. This work advances our understanding of a prevalent microbial interaction and its potential for biocontrol.