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<p>Gene expression of <i>Plasmodium falciparum</i> (<i>Pf</i>) liver-stage (LS) parasites has remained poorly characterized, although they are major vaccine and drug targets. Using a human liver-chimaeric mouse model and a fluorescent parasite line (<i>Pf</i>NF54<sup><i>CSP</i></sup>GFP), we isolated <i>Pf</i>LS and performed transcriptomics on key LS developmental phases. We linked clustered gene expression to ApiAP2, a major family of transcription factors that regulate the parasite life cycle. This provided insights into transcriptional regulation of LS infection and expression of essential LS metabolic and biosynthetic pathways. We observed expression of antigenically variant PfEMP1 proteins and the major <i>Pf</i> protein export machine PTEX and identified protein candidates that might be exported by LS parasites. Comparing <i>Pf</i> and <i>P. vivax</i> LS transcriptomes, we uncovered differences in their expression of sexual commitment factors. This data will aid LS research and vaccine and drug target identification for prevention of malaria infection.</p>

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Genome-wide gene expression profiles throughout human malaria parasite liver stage development in humanized mice

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年1月31日

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  • Gigliola Zanghí,
  • Hardik Patel,
  • Jenny L. Smith,
  • Nelly Camargo,
  • Yeji Bae,
  • Eva Hesping,
  • Justin A. Boddey,
  • Kannan Venugopal,
  • Matthias Marti,
  • Erika L. Flannery,
  • Vorada Chuenchob,
  • Matthew E. Fishbaugher,
  • Sebastian A. Mikolajczak,
  • Wanlapa Roobsoong,
  • Jetsumon Sattabongkot,
  • Priya Gupta,
  • Lucia Pazzagli,
  • Nastaran Rezakhani,
  • William Betz,
  • Kiera Hayes,
  • Debashree Goswami,
  • Ashley M. Vaughan,
  • Stefan H. I. Kappe

摘要

Gene expression of Plasmodium falciparum (Pf) liver-stage (LS) parasites has remained poorly characterized, although they are major vaccine and drug targets. Using a human liver-chimaeric mouse model and a fluorescent parasite line (PfNF54CSPGFP), we isolated PfLS and performed transcriptomics on key LS developmental phases. We linked clustered gene expression to ApiAP2, a major family of transcription factors that regulate the parasite life cycle. This provided insights into transcriptional regulation of LS infection and expression of essential LS metabolic and biosynthetic pathways. We observed expression of antigenically variant PfEMP1 proteins and the major Pf protein export machine PTEX and identified protein candidates that might be exported by LS parasites. Comparing Pf and P. vivax LS transcriptomes, we uncovered differences in their expression of sexual commitment factors. This data will aid LS research and vaccine and drug target identification for prevention of malaria infection.