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<p>Green leaf volatiles (GLVs) are six-carbon volatile organic compounds that mediate plant responses to environmental stresses. The quantity and composition of emitted GLVs can vary with stress type, allowing plants to fine-tune their volatile blends. In addition, insect herbivores are capable of modulating these emissions. A key mechanism underlying this plasticity is the conversion of <i>Z</i>-3-hexenal to <i>E</i>-2-hexenal by the enzyme (3<i>Z</i>):(2<i>E</i>)-hexenal isomerase (Hi), which reshapes GLV profiles and may influence multitrophic interactions. Here we investigate the evolutionary origin, functional diversification and catalytic mechanisms of lepidopteran Hi homologues, which belong to the glucose–methanol–choline oxidoreductase family. Phylogenetic analysis of 34 lepidopteran species identified a distinct glucose–methanol–choline-β subclade enriched in Hi homologues, largely confined to the Apoditrysia lineage. Functional assays showed species-specific variation in Hi activity, with <i>Manduca sexta</i> Hi-1 displaying the highest activity among tested homologues under identical protein concentrations, both in vitro and in planta. Structural modelling and site-directed mutagenesis revealed that Hi activity requires a flavin adenine dinucleotide cofactor enabling the identification of key residues critical for flavin adenine dinucleotide binding. Comparative phylogenetics further suggests that Hi enzymes in plants and Lepidoptera evolved independently from unrelated enzyme families, representing a case of functional convergence coinciding with the Cretaceous angiosperm radiation.</p>

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Convergent evolution of hexenal isomerases in Lepidoptera and plants

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月27日

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  • Yu-Hsien Lin,
  • Bulah Chia-hsiang Wu,
  • Abdoallah Sharaf,
  • Sophie Maartje Elisabeth Heijblom,
  • Ilias Prattis,
  • Ching-Wen Tan,
  • Rudolf J. Schilder,
  • Jared Gregory Ali,
  • Silke Allmann

摘要

Green leaf volatiles (GLVs) are six-carbon volatile organic compounds that mediate plant responses to environmental stresses. The quantity and composition of emitted GLVs can vary with stress type, allowing plants to fine-tune their volatile blends. In addition, insect herbivores are capable of modulating these emissions. A key mechanism underlying this plasticity is the conversion of Z-3-hexenal to E-2-hexenal by the enzyme (3Z):(2E)-hexenal isomerase (Hi), which reshapes GLV profiles and may influence multitrophic interactions. Here we investigate the evolutionary origin, functional diversification and catalytic mechanisms of lepidopteran Hi homologues, which belong to the glucose–methanol–choline oxidoreductase family. Phylogenetic analysis of 34 lepidopteran species identified a distinct glucose–methanol–choline-β subclade enriched in Hi homologues, largely confined to the Apoditrysia lineage. Functional assays showed species-specific variation in Hi activity, with Manduca sexta Hi-1 displaying the highest activity among tested homologues under identical protein concentrations, both in vitro and in planta. Structural modelling and site-directed mutagenesis revealed that Hi activity requires a flavin adenine dinucleotide cofactor enabling the identification of key residues critical for flavin adenine dinucleotide binding. Comparative phylogenetics further suggests that Hi enzymes in plants and Lepidoptera evolved independently from unrelated enzyme families, representing a case of functional convergence coinciding with the Cretaceous angiosperm radiation.