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<p>Light-responsive proteins play an essential role in all domains of life by sensing and responding to environmental light signals. However, the de novo design of light-responsive proteins with precisely defined structures and reversible responsive behaviours is an unmet challenge. Here we describe a computational approach to design protein–protein interactions regulated by non-canonical amino acids, focusing on the light-responsive phenylalanine-4′-azobenzene (AzoF). Using this approach, we designed light-responsive cyclic homo-oligomers and heterodimers, which only assemble in AzoF’s <i>trans</i> configuration and disassemble when AzoF photoisomerizes to the <i>cis</i> configuration. Biophysical characterization confirms the light-responsive assembly and disassembly of these complexes, and the crystal structures match the design models with atomic accuracy. We demonstrate the applicability of these light-responsive proteins in constructing light-responsive hydrogels and engineering synthetic ligand receptors to optocontrol cell signalling in mammalian cells. Our approach opens avenues for designing environmentally responsive protein structures and broadens the toolkit for optogenetics and optochemistry.</p><p></p>

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De novo design of light-responsive protein–protein interactions enables reversible formation of protein assemblies

  • 期刊论文

  • 发表日期：

    2025年8月28日

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  • Bowen Yu,
  • Jiao Liu,
  • Zhanyuan Cui,
  • Chu Wang,
  • Peipei Chen,
  • Chentong Wang,
  • Yanzhe Zhang,
  • Xingxing Zhu,
  • Ze Zhang,
  • Shichao Li,
  • Jinheng Pan,
  • Mingqi Xie,
  • Huaizong Shen,
  • Longxing Cao

摘要

Light-responsive proteins play an essential role in all domains of life by sensing and responding to environmental light signals. However, the de novo design of light-responsive proteins with precisely defined structures and reversible responsive behaviours is an unmet challenge. Here we describe a computational approach to design protein–protein interactions regulated by non-canonical amino acids, focusing on the light-responsive phenylalanine-4′-azobenzene (AzoF). Using this approach, we designed light-responsive cyclic homo-oligomers and heterodimers, which only assemble in AzoF’s trans configuration and disassemble when AzoF photoisomerizes to the cis configuration. Biophysical characterization confirms the light-responsive assembly and disassembly of these complexes, and the crystal structures match the design models with atomic accuracy. We demonstrate the applicability of these light-responsive proteins in constructing light-responsive hydrogels and engineering synthetic ligand receptors to optocontrol cell signalling in mammalian cells. Our approach opens avenues for designing environmentally responsive protein structures and broadens the toolkit for optogenetics and optochemistry.