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<p>Successful surgical resection of solid tumours requires highly reliable real-time intraoperative tools to accurately delineate tumour boundaries, which remains challenging in routine clinical standards. Here, we identify endogenous substances with intense autofluorescence in the second near-infrared window (NIR-II, 1,000–1,700 nm) that are abundant in human liver tissues but negligible in cancerous tissues. Inspired by this discovery, we develop a label-free and wide-field imaging approach, named tissue autofluorescence NIR-II imaging (TANI) for visualizing human liver malignancies. TANI demonstrates exceptional contrast (7.69 ± 0.52), sensitivity (97.8%) and specificity (98.4%) in delineating various liver malignancies, including hepatocellular carcinoma, intrahepatic cholangiocarcinoma and liver metastasis from cirrhotic or non-cirrhotic livers, outperforming routine fluorescence-guided surgery and conventional autofluorescence imaging in the visible (400–650 nm) or first near-infrared (700–900 nm) window. The excellent performance of TANI remains unaffected by cancer grade/stage, benign lesions or blood/bile contamination. These findings represent a promising advance in intraoperative decision-making and suggest a strong correlation between near-infrared autofluorescence and diseases. We believe that clarifying the molecular insights underlying these autofluorescent substances may provide new diagnostic directions.</p>

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Label-free tissue NIR-II autofluorescence imaging for visualization of human liver malignancy

  • 期刊论文

  • 发表日期：

    2026年1月20日

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  • Haisheng He,
  • Wenwei Zhu,
  • Han Miao,
  • Shangfeng Wang,
  • Zunguo Du,
  • Hongxin Zhang,
  • Jiang Ming,
  • Ben Shi,
  • Hao Wang,
  • Jianping Qi,
  • Yong Fan,
  • Wei Wu,
  • Dongyuan Zhao,
  • Lun-Xiu Qin,
  • Fan Zhang

摘要

Successful surgical resection of solid tumours requires highly reliable real-time intraoperative tools to accurately delineate tumour boundaries, which remains challenging in routine clinical standards. Here, we identify endogenous substances with intense autofluorescence in the second near-infrared window (NIR-II, 1,000–1,700 nm) that are abundant in human liver tissues but negligible in cancerous tissues. Inspired by this discovery, we develop a label-free and wide-field imaging approach, named tissue autofluorescence NIR-II imaging (TANI) for visualizing human liver malignancies. TANI demonstrates exceptional contrast (7.69 ± 0.52), sensitivity (97.8%) and specificity (98.4%) in delineating various liver malignancies, including hepatocellular carcinoma, intrahepatic cholangiocarcinoma and liver metastasis from cirrhotic or non-cirrhotic livers, outperforming routine fluorescence-guided surgery and conventional autofluorescence imaging in the visible (400–650 nm) or first near-infrared (700–900 nm) window. The excellent performance of TANI remains unaffected by cancer grade/stage, benign lesions or blood/bile contamination. These findings represent a promising advance in intraoperative decision-making and suggest a strong correlation between near-infrared autofluorescence and diseases. We believe that clarifying the molecular insights underlying these autofluorescent substances may provide new diagnostic directions.