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<p>The intricate topology of vascular networks and the complex functions of vessel-rich tissues are challenging to reconstruct in vitro. Here we report the development of: in vitro pathological models of erectile dysfunction and Peyronie’s disease; a model of the penis that includes the glans and the corpus spongiosum with urethral structures; and an implantable model of the corpus cavernosum, whose complex vascular network is critical for erectile function, via the vein-occlusion effect. Specifically, we 3D printed a hydrogel-based corpus cavernosum incorporating a strain-limiting tunica albuginea that can be engorged with blood through vein occlusion. In corpus cavernosum defects in rabbits and pigs, implantation of the 3D-printed tissue seeded with endothelial cells restored normal erectile function on electrical stimulation of the cavernous nerves as well as spontaneous erectile function within a few weeks of implantation, which allowed the animals to mate and reproduce. Our findings support the further development of 3D-printed blood-vessel-rich functional organs for transplantation.</p>

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3D-printed perfused models of the penis for the study of penile physiology and for restoring erectile function in rabbits and pigs

  • 期刊论文

  • 发表日期：

    2025年3月4日

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  • Zhenxing Wang,
  • Xuemin Liu,
  • Tan Ye,
  • Zhichen Zhai,
  • Kai Wu,
  • Yudi Kuang,
  • Serge Ostrovidov,
  • Dan Shao,
  • Yingjun Wang,
  • Kam W. Leong,
  • Xuetao Shi

摘要

The intricate topology of vascular networks and the complex functions of vessel-rich tissues are challenging to reconstruct in vitro. Here we report the development of: in vitro pathological models of erectile dysfunction and Peyronie’s disease; a model of the penis that includes the glans and the corpus spongiosum with urethral structures; and an implantable model of the corpus cavernosum, whose complex vascular network is critical for erectile function, via the vein-occlusion effect. Specifically, we 3D printed a hydrogel-based corpus cavernosum incorporating a strain-limiting tunica albuginea that can be engorged with blood through vein occlusion. In corpus cavernosum defects in rabbits and pigs, implantation of the 3D-printed tissue seeded with endothelial cells restored normal erectile function on electrical stimulation of the cavernous nerves as well as spontaneous erectile function within a few weeks of implantation, which allowed the animals to mate and reproduce. Our findings support the further development of 3D-printed blood-vessel-rich functional organs for transplantation.