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<p>The anoxic-oxic (A/O) process is the most common biological method for removing nitrogen (N) from wastewater, but the antibiotic resistance profile of N-metabolizing microbes in A/O processes remains largely underexplored. Here we demonstrated a significant positive correlation between various types of N-metabolizing genes and antibiotic resistance genes (ARGs) in swine wastewater A/O processes across China. We assembled 180 high-quality genomes of dominant N-metabolizing microbes (12.6% of the total metagenome-assembled genomes), all harboring transcriptionally active ARGs. And Pseudomonas was identified as the primary N-metabolizing genus and major ARG host. Among 1110 culturable N-metabolizing isolates, 22.34% were Pseudomonas strains showing high N removal capacity and multi-antibiotic resistance. Moreover, plasmid-mediated ARG transfer further heightened resistance risks. Overall, these findings highlight a significant ARG risk among predominant N-metabolizing microbes in A/O treatment processes, underscoring the urgency of balancing N removal performance with resistance control in wastewater treatment processes.</p>

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Antibiotic resistance profile of nitrogen-metabolizing microbes in anoxic‒oxic processes for swine wastewater treatment

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月20日

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  • Yiwen Yang,
  • Shuang Cai,
  • Feng Huang,
  • Chunhao Mo,
  • Yongbao Wu,
  • Junting Cao,
  • Sheng Chen,
  • Zhiguo Wen,
  • Xindi Liao

摘要

The anoxic-oxic (A/O) process is the most common biological method for removing nitrogen (N) from wastewater, but the antibiotic resistance profile of N-metabolizing microbes in A/O processes remains largely underexplored. Here we demonstrated a significant positive correlation between various types of N-metabolizing genes and antibiotic resistance genes (ARGs) in swine wastewater A/O processes across China. We assembled 180 high-quality genomes of dominant N-metabolizing microbes (12.6% of the total metagenome-assembled genomes), all harboring transcriptionally active ARGs. And Pseudomonas was identified as the primary N-metabolizing genus and major ARG host. Among 1110 culturable N-metabolizing isolates, 22.34% were Pseudomonas strains showing high N removal capacity and multi-antibiotic resistance. Moreover, plasmid-mediated ARG transfer further heightened resistance risks. Overall, these findings highlight a significant ARG risk among predominant N-metabolizing microbes in A/O treatment processes, underscoring the urgency of balancing N removal performance with resistance control in wastewater treatment processes.