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<p>To address suboptimal antibody induction by commercial PEDV vaccines, we constructed a recombinant adeno-associated virus (rAAV-CMV-PEDV S1) expressing regions of the PEDV S1 subunit based on Adeno-associated virus serotype 2 (AAV-2) vector. In mice, rAAV-CMV-PEDV S1 induced significantly more remarkable and persistent serum-specific IgG compared with the inactivated vaccine and accompanied by robust CD3<sup>+</sup>CD8<sup>+</sup> T cells activation within 4 weeks post-immunization. In pregnant sows, compared with commercially available inactivated and attenuated vaccines, the rAAV-CMV-PEDV S1 induced significantly higher serum and colostral IgG as well as neutralizing antibody titers, which provided piglets with abundant maternal antibodies. Crucially, challenge experiments demonstrated that rAAV-CMV-PEDV S1 conferred 80% clinical protective efficacy for piglets, accompanied by significantly reduced viral shedding loads, surpassing other vaccine groups. These findings suggest that rAAV-CMV-PEDV S1 candidate could be a promising PEDV vaccine for enhancing antibody levels and could strengthen the protection of piglets against PEDV infection.</p>

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Recombinant AAV vaccine expressing PEDV S1 enhances immunity with superior neonatal protection

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月6日

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  • Maonan Pang,
  • Meishen Ren,
  • Dike Jiang,
  • Teng Tu,
  • You Zhou,
  • Yin Wang

摘要

To address suboptimal antibody induction by commercial PEDV vaccines, we constructed a recombinant adeno-associated virus (rAAV-CMV-PEDV S1) expressing regions of the PEDV S1 subunit based on Adeno-associated virus serotype 2 (AAV-2) vector. In mice, rAAV-CMV-PEDV S1 induced significantly more remarkable and persistent serum-specific IgG compared with the inactivated vaccine and accompanied by robust CD3+CD8+ T cells activation within 4 weeks post-immunization. In pregnant sows, compared with commercially available inactivated and attenuated vaccines, the rAAV-CMV-PEDV S1 induced significantly higher serum and colostral IgG as well as neutralizing antibody titers, which provided piglets with abundant maternal antibodies. Crucially, challenge experiments demonstrated that rAAV-CMV-PEDV S1 conferred 80% clinical protective efficacy for piglets, accompanied by significantly reduced viral shedding loads, surpassing other vaccine groups. These findings suggest that rAAV-CMV-PEDV S1 candidate could be a promising PEDV vaccine for enhancing antibody levels and could strengthen the protection of piglets against PEDV infection.