Scholarlink

<p><i>Isatis indigotica</i> fruit is an underutilized source of natural antioxidant ingredients. However, the developmental basis of its bioactive metabolites remains unclear. We integrated widely targeted metabolomics, transcriptomics, and antioxidant phenotyping across five fruit developmental stages (S1–S5) to characterize metabolic remodeling, identify flavonoid-related metabolite features associated with antioxidant capacity, and infer candidate regulatory modules. We detected 2413 metabolites, revealing pronounced developmental reprogramming and late-stage enrichment of flavonoid-related metabolites, particularly anthocyanin-associated features. Consistent with these changes, DPPH 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, FRAP (ferric reducing antioxidant power), ABTS (2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)) radical scavenging activity, and total flavonoid content increased markedly during maturation, reaching 4.5-, 7.1-, 4.6-, and 13.0-fold of the S1 levels, respectively. Correlation analysis prioritized 12 flavonoid-related metabolite features strongly associated with antioxidant indices. Integrated transcriptomic analysis and weighted gene co-expression network analysis further identified a co-expression module linked to these features and highlighted Iin17052 (NAC family), Iin08518 (C3H family), and Iin25007 (MYB family) transcription factors as candidate regulators associated with late flavonoid biosynthetic genes, including <i>DIHYDROFLAVONOL 4-REDUCTASE</i> and <i>ANTHOCYANIDIN SYNTHASE</i>. Together, these findings provide a stage-resolved framework for harvest-stage selection and support the valorization of <i>I. indigotica</i> fruit as a potential source of plant-derived antioxidant ingredients for functional food development.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Stage-resolved multi-omics links flavonoid accumulation to antioxidant capacity in Isatis indigotica fruit

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年7月20日

查看全文
  • Rong Chen,
  • Xiaoshan Geng,
  • Lili Wang,
  • Xuexue Wang,
  • Fanglin Liu,
  • Yude Peng,
  • Chunfeng Tang,
  • Huaxi Huang,
  • Qin Liu

摘要

Isatis indigotica fruit is an underutilized source of natural antioxidant ingredients. However, the developmental basis of its bioactive metabolites remains unclear. We integrated widely targeted metabolomics, transcriptomics, and antioxidant phenotyping across five fruit developmental stages (S1–S5) to characterize metabolic remodeling, identify flavonoid-related metabolite features associated with antioxidant capacity, and infer candidate regulatory modules. We detected 2413 metabolites, revealing pronounced developmental reprogramming and late-stage enrichment of flavonoid-related metabolites, particularly anthocyanin-associated features. Consistent with these changes, DPPH 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, FRAP (ferric reducing antioxidant power), ABTS (2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)) radical scavenging activity, and total flavonoid content increased markedly during maturation, reaching 4.5-, 7.1-, 4.6-, and 13.0-fold of the S1 levels, respectively. Correlation analysis prioritized 12 flavonoid-related metabolite features strongly associated with antioxidant indices. Integrated transcriptomic analysis and weighted gene co-expression network analysis further identified a co-expression module linked to these features and highlighted Iin17052 (NAC family), Iin08518 (C3H family), and Iin25007 (MYB family) transcription factors as candidate regulators associated with late flavonoid biosynthetic genes, including DIHYDROFLAVONOL 4-REDUCTASE and ANTHOCYANIDIN SYNTHASE. Together, these findings provide a stage-resolved framework for harvest-stage selection and support the valorization of I. indigotica fruit as a potential source of plant-derived antioxidant ingredients for functional food development.