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<p>Based on the specific aptamer-functionalized magnetic beads and the hybrid chain reaction (HCR), a rapid detection method for <i>Bacillus cereus</i> was established. <i>B. cereus</i> competes with carboxylfluorescein-labeled cDNA (FAM-cDNA) to bind to biotin-labeled aptamer fixed on streptavidin magnetic beads. After magnetic separation, the released FAM-cDNA was used as an initiator to trigger HCR amplification, significantly enhancing the fluorescence signal. In the absence of <i>B. cereus</i>, the initiator binds to the aptamer, generating only a weak fluorescent signal. More importantly, graphene oxide (GO) can quench the fluorescence of unreacted FAM-cDNA, achieving a more accurate detection effect and thus enabling the detection of <i>B. cereus</i> at lower concentrations. This method has good specificity and can detect <i>B. cereus</i> as low as 5.4×10<sup>1</sup> CFU/mL. A linear response was observed across the concentration range of 5.4×10<sup>5</sup> CFU/mL-5.4×10<sup>1</sup> CFU/mL. The feasibility of this method was verified by detecting <i>B. cereus</i> in artificially contaminated milk samples.</p><p></p>

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Aptamer-functionalized magnetic beads combined with hybridization chain reaction for detection of Bacillus cereus

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年3月2日

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  • Xiaoting Song,
  • Changzheng Shi,
  • Yunbin Lv,
  • Xinmei Liu,
  • Xiaomei Bie,
  • Jun Yang

摘要

Based on the specific aptamer-functionalized magnetic beads and the hybrid chain reaction (HCR), a rapid detection method for Bacillus cereus was established. B. cereus competes with carboxylfluorescein-labeled cDNA (FAM-cDNA) to bind to biotin-labeled aptamer fixed on streptavidin magnetic beads. After magnetic separation, the released FAM-cDNA was used as an initiator to trigger HCR amplification, significantly enhancing the fluorescence signal. In the absence of B. cereus, the initiator binds to the aptamer, generating only a weak fluorescent signal. More importantly, graphene oxide (GO) can quench the fluorescence of unreacted FAM-cDNA, achieving a more accurate detection effect and thus enabling the detection of B. cereus at lower concentrations. This method has good specificity and can detect B. cereus as low as 5.4×101 CFU/mL. A linear response was observed across the concentration range of 5.4×105 CFU/mL-5.4×101 CFU/mL. The feasibility of this method was verified by detecting B. cereus in artificially contaminated milk samples.