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<p>This study evaluates the feasibility of producing nozzle-less eugenol-infused gelatin nanofibers as active coatings to improve fish quality and shelf life during cold storage. Nanofibers were fabricated using nozzle-less electrospinning with gelatin concentrations of 10–20% and gelatin:eugenol ratios of 100:0 to 50:50. The optimal formulation—20% gelatin with a 50:50 ratio—achieved &gt;99.98% encapsulation efficiency and the smallest average fiber diameter (91.11 ± 18.53 nm). FTIR confirmed strong hydrogen bonding between gelatin and eugenol, while TGA indicated improved thermal stability. Higher eugenol loading enhanced antioxidant activity, limiting lipid oxidation to &lt;0.15 mg MDA/kg compared with &gt;1 mg MDA/kg in controls after 3 days. Microbial counts in coated fish at day 7 (2.80 log CFU/g) remained lower than uncoated samples at day 3 (3.88 log CFU/g). The coating also preserved texture and color throughout storage. Overall, these findings highlight the potential of bioactive nanofiber coatings for food preservation and waste reduction.</p>

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Nozzle-less electrospun eugenol-loaded gelatin nanofibers: effect on fish preservation and quality enhancement

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年1月9日

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  • Parya Shirmohammadi,
  • Nafiseh Soltanizadeh,
  • Milad Fathi,
  • Alireza Allafchian

摘要

This study evaluates the feasibility of producing nozzle-less eugenol-infused gelatin nanofibers as active coatings to improve fish quality and shelf life during cold storage. Nanofibers were fabricated using nozzle-less electrospinning with gelatin concentrations of 10–20% and gelatin:eugenol ratios of 100:0 to 50:50. The optimal formulation—20% gelatin with a 50:50 ratio—achieved >99.98% encapsulation efficiency and the smallest average fiber diameter (91.11 ± 18.53 nm). FTIR confirmed strong hydrogen bonding between gelatin and eugenol, while TGA indicated improved thermal stability. Higher eugenol loading enhanced antioxidant activity, limiting lipid oxidation to <0.15 mg MDA/kg compared with >1 mg MDA/kg in controls after 3 days. Microbial counts in coated fish at day 7 (2.80 log CFU/g) remained lower than uncoated samples at day 3 (3.88 log CFU/g). The coating also preserved texture and color throughout storage. Overall, these findings highlight the potential of bioactive nanofiber coatings for food preservation and waste reduction.