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<p>Transportation, a critical link in the aquaculture industry chain, triggers significant economic losses through stress-induced muscle quality deterioration in fish. This multi-omics study explored the mechanisms underlying transportation stress-induced quality deterioration of fish muscle. Transportation stress elevated cortisol, glucose, lactate dehydrogenase, and oxidative markers (SOD), alongside gill/liver tissue damage. Stress reduced muscle shear force, whiteness, and water-holding capacity, which were highly related to the disruption of muscular structure. Transcriptomics revealed dysregulation of AMPK, PI3K-Akt, FoxO, and MAPK pathways <i>via pfkfb</i>, <i>akt</i>, <i>gadd45</i>, <i>gabarap</i>, <i>jun</i>. Metabolomics highlighted TCA cycle disruption, altered fructose and mannose/pyruvate metabolism, and purine imbalance (e.g., D-mannose 6-phosphate, malate, IMP). Muscle quality parameters negatively correlated with oxidative stress (<i>gpx</i>) and apoptosis (<i>casp3/8/9</i>), but positively linked to DL-glutamine and D-fructose 6-phosphate. Results demonstrate that transportation stress impairs muscle quality <i>via</i> energy dysregulation, oxidative damage, and apoptosis, thus providing a theoretical basis to optimize aquaculture transport and reduce economic losses.</p><p></p>

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Comprehensive transcriptomic and metabolomic analysis provides insight into transportation stress effects on fish muscle quality

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年1月7日

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  • Yujie Meng,
  • Liangzi Zhang,
  • Shikang Sang,
  • Zenan Huang,
  • Ru Liu,
  • Juan You,
  • Qilin Huang,
  • Yong Long,
  • Guili Song,
  • Dan Jia,
  • Tao Yin

摘要

Transportation, a critical link in the aquaculture industry chain, triggers significant economic losses through stress-induced muscle quality deterioration in fish. This multi-omics study explored the mechanisms underlying transportation stress-induced quality deterioration of fish muscle. Transportation stress elevated cortisol, glucose, lactate dehydrogenase, and oxidative markers (SOD), alongside gill/liver tissue damage. Stress reduced muscle shear force, whiteness, and water-holding capacity, which were highly related to the disruption of muscular structure. Transcriptomics revealed dysregulation of AMPK, PI3K-Akt, FoxO, and MAPK pathways via pfkfb, akt, gadd45, gabarap, jun. Metabolomics highlighted TCA cycle disruption, altered fructose and mannose/pyruvate metabolism, and purine imbalance (e.g., D-mannose 6-phosphate, malate, IMP). Muscle quality parameters negatively correlated with oxidative stress (gpx) and apoptosis (casp3/8/9), but positively linked to DL-glutamine and D-fructose 6-phosphate. Results demonstrate that transportation stress impairs muscle quality via energy dysregulation, oxidative damage, and apoptosis, thus providing a theoretical basis to optimize aquaculture transport and reduce economic losses.