Scholarlink

<p>Bacteria-assisted click chemistry for enhanced nanozyme-based <i>Escherichia coli</i> (<i>E. coli</i>) detection. The size and shape of the alkyne-modified Fe<sub>3</sub>O<sub>4</sub>@Au NPs were 110 nm and urchin-like, respectively. The azide-functionalized Au QDs were spherical in shape, and their size was ~5 nm. The absorbance and emission peaks of Fe<sub>3</sub>O<sub>4</sub>@Au NPs and Au QDs were located at 526 nm and 520 nm, respectively. The nanozymatic activity of both materials significantly improved in the presence of Cu<sup>2+</sup>, <i>E</i>. coli, and 3,3′,5,5′-tetramethylbenzidine (TMBZ)/H<sub>2</sub>O<sub>2</sub> mixture. Here, the bacterial metabolic product Cu<sup>+</sup> was utilized to trigger the click reaction between alkyne-modified Fe<sub>3</sub>O<sub>4</sub>@Au NPs and azide-functionalized Au QDs which enables magnetic separation. The calculated limits of detection (LODs) of <i>E. coli</i> were as high as 5 CFU/mL and 25 CFU/mL in the PBS buffer and spiked raw meat samples, respectively. The proposed <i>nanozymatic E. coli</i> assay was selective in the presence of other bacteria.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A novel strategy for Escherichia coli detection in raw beef in combination with click chemistry

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年4月24日

查看全文
  • Abdulhakeem Alzahrani

摘要

Bacteria-assisted click chemistry for enhanced nanozyme-based Escherichia coli (E. coli) detection. The size and shape of the alkyne-modified Fe3O4@Au NPs were 110 nm and urchin-like, respectively. The azide-functionalized Au QDs were spherical in shape, and their size was ~5 nm. The absorbance and emission peaks of Fe3O4@Au NPs and Au QDs were located at 526 nm and 520 nm, respectively. The nanozymatic activity of both materials significantly improved in the presence of Cu2+, E. coli, and 3,3′,5,5′-tetramethylbenzidine (TMBZ)/H2O2 mixture. Here, the bacterial metabolic product Cu+ was utilized to trigger the click reaction between alkyne-modified Fe3O4@Au NPs and azide-functionalized Au QDs which enables magnetic separation. The calculated limits of detection (LODs) of E. coli were as high as 5 CFU/mL and 25 CFU/mL in the PBS buffer and spiked raw meat samples, respectively. The proposed nanozymatic E. coli assay was selective in the presence of other bacteria.