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<p>Co-occurrence of multiple mycotoxins is a growing global food safety concern due to their harmful effects on humans and animals. This study developed an eco-friendly sample preparation method and an innovative multiplex microarray-based lateral flow immunoassay, using a novel portable reader for on-site simultaneous determination of five regulated mycotoxins—aflatoxin B<sub>1</sub>, T-2 toxin, zearalenone, deoxynivalenol, and fumonisin B<sub>1</sub> in rice. The eco-friendly and ultrafast extraction procedure utilizes a bio-based solvent. Principally, the microarray signals generated through a novel luminescent organic dye were captured to quantify mycotoxin levels in samples using a portable reader installed with a user-friendly interface. The assay demonstrates accurate detection and quantification of these mycotoxins, with recoveries ranging from 77% to 127%. Detection limits ranged from 0.56 to 1.89 μg/kg, which were well below their regulatory limits, with a relative standard deviation below 25%. This analytical system provides an on-site method for detecting multiple mycotoxins in rice.</p>

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A multiplex microarray lateral flow immunoassay device for simultaneous determination of five mycotoxins in rice

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2024年12月31日

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  • Saowalak Adunphatcharaphon,
  • Oluwatobi Kolawole,
  • Thanasat Sooksimuang,
  • Waraporn Panchan,
  • Wannee Wasuthep,
  • Anuwut Petdum,
  • Grit Pichayawaytin,
  • Rungroj Jintamethasawat,
  • Narusorn Doljirapisit,
  • Armote Somboonkaew,
  • Prae Noppakuadrittidej,
  • Sudtida Kaew-amdee,
  • Manlika Makornwattana,
  • Julie Meneely,
  • Christopher T. Elliott,
  • Awanwee Petchkongkaew,
  • Nitsara Karoonuthaisiri

摘要

Co-occurrence of multiple mycotoxins is a growing global food safety concern due to their harmful effects on humans and animals. This study developed an eco-friendly sample preparation method and an innovative multiplex microarray-based lateral flow immunoassay, using a novel portable reader for on-site simultaneous determination of five regulated mycotoxins—aflatoxin B1, T-2 toxin, zearalenone, deoxynivalenol, and fumonisin B1 in rice. The eco-friendly and ultrafast extraction procedure utilizes a bio-based solvent. Principally, the microarray signals generated through a novel luminescent organic dye were captured to quantify mycotoxin levels in samples using a portable reader installed with a user-friendly interface. The assay demonstrates accurate detection and quantification of these mycotoxins, with recoveries ranging from 77% to 127%. Detection limits ranged from 0.56 to 1.89 μg/kg, which were well below their regulatory limits, with a relative standard deviation below 25%. This analytical system provides an on-site method for detecting multiple mycotoxins in rice.