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<p>The diesel fungus <i>Amorphotheca resinae</i> can influence corrosion in fuel infrastructure. We compared six isolates in their interaction with carbon steel using two carbon sources, glucose and a 7% biodiesel (B7) blend. All isolates accelerated uniform corrosion with glucose, while with biodiesel, <i>A. resinae</i> had no effect on uniform corrosion but inhibited localised corrosion. The secretion of a melanin-like polymer occurred only in the presence of glucose and carbon steel. Using CRISPR/Cas9-mediated genome editing, we generated and tested melanin-deficient (Δ<i>pks1</i>, Δ<i>cmr1</i>) and constitutive melanin-producing strains of <i>A. resinae</i>. The localised corrosion rates were reduced for melanin-deficient mutants, while the uniform corrosion rates remained unchanged. Addition of secreted melanin to abiotic set-ups increased the uniform corrosion rate. These findings suggest that melanin (cell wall-bound and/or secreted) promotes localised corrosion, whereas secreted melanin accelerates uniform corrosion. The fungus-steel interaction depends both on the cellular physiology and the carbon source, highlighting the complicated role of fungi in carbon steel corrosion.</p>

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The role of a melanin-like polymer in carbon steel corrosion by Amorphotheca resinae

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月20日

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  • Ruben Gerrits,
  • Julia Schumacher,
  • Robert Prate,
  • Ines Feldmann,
  • Matthias Weise,
  • Anna A. Gorbushina,
  • Dennis Enning

摘要

The diesel fungus Amorphotheca resinae can influence corrosion in fuel infrastructure. We compared six isolates in their interaction with carbon steel using two carbon sources, glucose and a 7% biodiesel (B7) blend. All isolates accelerated uniform corrosion with glucose, while with biodiesel, A. resinae had no effect on uniform corrosion but inhibited localised corrosion. The secretion of a melanin-like polymer occurred only in the presence of glucose and carbon steel. Using CRISPR/Cas9-mediated genome editing, we generated and tested melanin-deficient (Δpks1, Δcmr1) and constitutive melanin-producing strains of A. resinae. The localised corrosion rates were reduced for melanin-deficient mutants, while the uniform corrosion rates remained unchanged. Addition of secreted melanin to abiotic set-ups increased the uniform corrosion rate. These findings suggest that melanin (cell wall-bound and/or secreted) promotes localised corrosion, whereas secreted melanin accelerates uniform corrosion. The fungus-steel interaction depends both on the cellular physiology and the carbon source, highlighting the complicated role of fungi in carbon steel corrosion.