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<p>Polypropylene (PP) is the second most abundantly used synthetic plastic which poses challenges in degradation due to its structural stability. There are limited reports on enzymatic biodegradation of PP. Here, we report degradation of commercial PP strips without any pre-treatment by a novel multi copper oxidase (MCO) from <i>Micrococcus</i> sp. IITD107. The molecular docking and simulation analysis revealed strong binding of MCO with PP. The purified enzyme and the whole cell lysate from recombinant <i>E. coli</i> expressing MCO, exhibited around 16% and 6.8% decrease (w/w), respectively in PP within 24 h at 60 ˚C. Changes in the functional groups and surface modification of PP were observed by FTIR, NMR and SEM. The biodegradation by-products identified were alkanes, aldehydes, ketones and a few carboxylic acids. This is the first report demonstrating the biodegradation of PP by MCO without any pre-treatment, suggesting this to have a significant role for PP degradation.</p>

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Multi copper oxidase from Micrococcus sp. IITD107, a promising candidate for polypropylene biodegradation

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年12月30日

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  • Poorvika Kulshrestha,
  • Richa Katiyar,
  • Arif Nissar Zargar,
  • Shoaib Haidar,
  • Pragya Kesarwani,
  • Milind Chavan,
  • Balaji Bandyopadhyay,
  • Debraj Chatterjee,
  • Durai Sundar,
  • Anurag S. Rathore,
  • Preeti Srivastava

摘要

Polypropylene (PP) is the second most abundantly used synthetic plastic which poses challenges in degradation due to its structural stability. There are limited reports on enzymatic biodegradation of PP. Here, we report degradation of commercial PP strips without any pre-treatment by a novel multi copper oxidase (MCO) from Micrococcus sp. IITD107. The molecular docking and simulation analysis revealed strong binding of MCO with PP. The purified enzyme and the whole cell lysate from recombinant E. coli expressing MCO, exhibited around 16% and 6.8% decrease (w/w), respectively in PP within 24 h at 60 ˚C. Changes in the functional groups and surface modification of PP were observed by FTIR, NMR and SEM. The biodegradation by-products identified were alkanes, aldehydes, ketones and a few carboxylic acids. This is the first report demonstrating the biodegradation of PP by MCO without any pre-treatment, suggesting this to have a significant role for PP degradation.