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<p>Understanding biocide performance in mixed-species biofilms is critical to mitigating microbiologically influenced corrosion (MIC). In this study, a novel dual anaerobic biofilm reactor was used to evaluate glutaraldehyde efficacy under environmentally relevant conditions, using a complex microbial consortium from marine sediment. Despite biocide dosing, biofilms persisted and induced localized corrosion, indicating incomplete mitigation. Each biocide application led to an electronegative shift in <i>E</i><sub>corr</sub> and a reduction in <InlineEquation ID="IEq1"> <InlineMediaObject> <ImageObject Color="BlackWhite" FileRef="41529_2025_628_Article_IEq1.gif" Format="GIF" Height="16" Rendition="HTML" Resolution="72" Type="Linedraw" Width="29" /> </InlineMediaObject> <EquationSource Format="TEX">\({{\rm{H}}}_{2}{\rm{S}}\)</EquationSource> <EquationSource Format="MATHML"><math> <mrow> <msub> <mrow> <mi mathvariant="normal">H</mi> </mrow> <mrow> <mn>2</mn> </mrow> </msub> <mi mathvariant="normal">S</mi> </mrow> </math></EquationSource> </InlineEquation> concentration, suggesting partial suppression of microbial activity. Raman spectroscopy and profilometry revealed differences in corrosion product composition and pit morphology between biotic and abiotic systems. 16S rRNA sequencing showed enrichment of stress-tolerant genera, including <i>Exiguobacterium</i> and <i>Serpentinicella</i>, consistent with increased chemical tolerance. These findings highlight the limitations of conventional biocide strategies and demonstrate the need for adaptive, community-informed treatment approaches. The dual-reactor model provides a robust platform for future MIC standardization efforts and mechanistic investigation of biofilm resilience under anoxic conditions.</p>

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Evaluating biocide efficacy in mixed-species biofilms: insights from a dual anaerobic biofilm reactor

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月31日

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  • Liam Jones,
  • Niall Hanrahan,
  • Maria Salta,
  • Torben Lund Skovhus,
  • Kathryn Thomas,
  • Timothy Illson,
  • Julian Wharton,
  • Jeremy Webb

摘要

Understanding biocide performance in mixed-species biofilms is critical to mitigating microbiologically influenced corrosion (MIC). In this study, a novel dual anaerobic biofilm reactor was used to evaluate glutaraldehyde efficacy under environmentally relevant conditions, using a complex microbial consortium from marine sediment. Despite biocide dosing, biofilms persisted and induced localized corrosion, indicating incomplete mitigation. Each biocide application led to an electronegative shift in Ecorr and a reduction in \({{\rm{H}}}_{2}{\rm{S}}\) H 2 S concentration, suggesting partial suppression of microbial activity. Raman spectroscopy and profilometry revealed differences in corrosion product composition and pit morphology between biotic and abiotic systems. 16S rRNA sequencing showed enrichment of stress-tolerant genera, including Exiguobacterium and Serpentinicella, consistent with increased chemical tolerance. These findings highlight the limitations of conventional biocide strategies and demonstrate the need for adaptive, community-informed treatment approaches. The dual-reactor model provides a robust platform for future MIC standardization efforts and mechanistic investigation of biofilm resilience under anoxic conditions.