Scholarlink

<p>Plants actively recruit beneficial rhizosphere microorganisms to enhance immunity, though the molecular mechanisms are largely unclear. Here we identified a complete <i>Os</i>MAPK–<i>Os</i>WRKY pathway of rice rhizosphere <i>Bacillus</i> recruitment via root secretion of heptadecanoic acid upon rice blast occurrence. In field trials, enrichment of <i>Bacillus</i> in their rhizospheres was associated with higher disease resistance of rice. Integrated omics analyses demonstrated a strong correlation between the activation of the <i>Os</i>MKK4–<i>Os</i>MPK6 cascade and <i>Bacillus</i> recruitment. Loss-of-function mutants of Os<i>MKK4</i> and Os<i>MPK6</i> reduce rhizosphere <i>Bacillus</i> abundance by 82% and 79%, respectively. As the substrates of <i>Os</i>MPK6, <i>Os</i>WRKY24/53/70 transcription factors activate Os<i>KCS2</i>, which encodes a β‑ketoacyl-CoA synthase involved in heptadecanoic acid biosynthesis. Mutations of Os<i>WRKY24/53/70</i> or Os<i>KCS2</i> reduce root-secreted heptadecanoic acid by 15–28% and impair <i>Bacillus</i> colonization. Heptadecanoic acid application recapitulated <i>Bacillus</i> recruitment and disease resistance. Collectively, our results demonstrate that the MAPK pathway regulates heptadecanoic acid synthesis, which controls <i>Bacillus</i> recruitment as well as rice blast disease resistance.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Rice roots recruit Bacillus via the secretion of heptadecanoic acid

  • 期刊论文

  • 发表日期：

    2026年4月28日

查看全文
  • Jianguo Zeng,
  • Tao Wen,
  • Jing Zhao,
  • Zihan Li,
  • Juan Zhang,
  • Guoqing Niu,
  • Yi Wen,
  • Weiye Liu,
  • Jianglan Cui,
  • Jinyang Zhang,
  • Qiuhui Yang,
  • Dongqing Shu,
  • Keyi Huang,
  • Wenyi Yang,
  • Tianjiao Zhang,
  • Zirui Chang,
  • Penghao Xie,
  • Mingfeng Feng,
  • Lei Wang,
  • Juan Xu,
  • Shuqun Zhang,
  • Qirong Shen,
  • Jun Yuan

摘要

Plants actively recruit beneficial rhizosphere microorganisms to enhance immunity, though the molecular mechanisms are largely unclear. Here we identified a complete OsMAPK–OsWRKY pathway of rice rhizosphere Bacillus recruitment via root secretion of heptadecanoic acid upon rice blast occurrence. In field trials, enrichment of Bacillus in their rhizospheres was associated with higher disease resistance of rice. Integrated omics analyses demonstrated a strong correlation between the activation of the OsMKK4–OsMPK6 cascade and Bacillus recruitment. Loss-of-function mutants of OsMKK4 and OsMPK6 reduce rhizosphere Bacillus abundance by 82% and 79%, respectively. As the substrates of OsMPK6, OsWRKY24/53/70 transcription factors activate OsKCS2, which encodes a β‑ketoacyl-CoA synthase involved in heptadecanoic acid biosynthesis. Mutations of OsWRKY24/53/70 or OsKCS2 reduce root-secreted heptadecanoic acid by 15–28% and impair Bacillus colonization. Heptadecanoic acid application recapitulated Bacillus recruitment and disease resistance. Collectively, our results demonstrate that the MAPK pathway regulates heptadecanoic acid synthesis, which controls Bacillus recruitment as well as rice blast disease resistance.