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<p><i>N</i><sup>6</sup>-methyladenosine (m<sup>6</sup>A) is the most prevalent internal modification on messenger RNA. Although recent studies have shown m<sup>6</sup>A effects on determining the fate of mRNA through modulating various aspects of plant mRNA metabolism, whether and how m<sup>6</sup>A affects gene transcription in plants remains elusive. Here we show that NEEDED FOR RDR2-INDEPENDENT DNA METHYLATION (NERD), a plant-specific protein, is an essential component of the m<sup>6</sup>A methyltransferase complex required for regulating the transcription of a central floral repressor <i>FLOWERING LOCUS C</i> (<i>FLC</i>) in <i>Arabidopsis</i>. NERD interacts with and stabilizes the two core methyltransferases, mRNA adenosine methylases A and B, to promote m<sup>6</sup>A modification of nascent RNA, conferring an overall negative effect on gene transcription. At the <i>FLC</i> locus, NERD-mediated m<sup>6</sup>A modification on the nascent transcript negatively affects H3K36me3 deposition and <i>FLC</i> transcription through NERD interaction with the H3K36me3 methyltransferase SET DOMAIN GROUP 8. Collectively, our findings reveal that NERD mediates the crosstalk between epitranscriptomic and epigenetic regulation of <i>FLC</i> to modulate flowering in <i>Arabidopsis</i>.</p>

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NERD-dependent m6A modification of the nascent FLC transcript regulates flowering time in Arabidopsis

  • 期刊论文

  • 发表日期：

    2025年3月14日

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  • Yanlin Shao,
  • Jinqi Ma,
  • Songyao Zhang,
  • Yifeng Xu,
  • Hao Yu

摘要

N6-methyladenosine (m6A) is the most prevalent internal modification on messenger RNA. Although recent studies have shown m6A effects on determining the fate of mRNA through modulating various aspects of plant mRNA metabolism, whether and how m6A affects gene transcription in plants remains elusive. Here we show that NEEDED FOR RDR2-INDEPENDENT DNA METHYLATION (NERD), a plant-specific protein, is an essential component of the m6A methyltransferase complex required for regulating the transcription of a central floral repressor FLOWERING LOCUS C (FLC) in Arabidopsis. NERD interacts with and stabilizes the two core methyltransferases, mRNA adenosine methylases A and B, to promote m6A modification of nascent RNA, conferring an overall negative effect on gene transcription. At the FLC locus, NERD-mediated m6A modification on the nascent transcript negatively affects H3K36me3 deposition and FLC transcription through NERD interaction with the H3K36me3 methyltransferase SET DOMAIN GROUP 8. Collectively, our findings reveal that NERD mediates the crosstalk between epitranscriptomic and epigenetic regulation of FLC to modulate flowering in Arabidopsis.