Scholarlink

<p>Cell-cycle progression is a major source of variability in live-cell phenotyping, yet imaging workflows still lack a general way to account for it alongside structural and functional readouts. Here we show CALIPERS (Cell-cycle-Aware Live-cell Imaging for Phenotyping Experiments and Regeneration Studies), an integrated framework that pairs a spectrally redesigned FUCCI reporter with continuous cell-cycle inference and flexible delivery strategies in human cells and stem-cell models. In epithelial cells, CALIPERS simultaneously images actin, tubulin, or calcium dynamics, phase-locks migration and proliferation, triggers mitosis-aware smart microscopy, and recovers fast calcium signals. In induced pluripotent stem-cell workflows, lentiviral and safe-harbor versions span pluripotent, lineage-restricted, and cardiac models. In cardiac organoids, CALIPERS tracks cell-cycle exit, tissue compaction, and calcium onset, and distinguishes productive proliferation from multinucleation and endoreplication. By making cell-cycle state an explicit, measurable variable, CALIPERS helps triage candidate regenerative interventions across diverse live-imaging assays.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

CALIPERS: Cell cycle-aware live imaging for phenotyping experiments and regeneration studies

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年8月1日

查看全文
  • Moises Di Sante,
  • Melissa Pezzotti,
  • Julius Zimmermann,
  • Alessandro Enrico,
  • Joran Deschamps,
  • Elisa Balmas,
  • Silvia Becca,
  • Eloisa Torchia,
  • Giulia Ballio,
  • Samantha Solito,
  • Martina Sarchi,
  • Alessandro Reali,
  • Alessandro Bertero,
  • Florian Jug,
  • Francesco S. Pasqualini

摘要

Cell-cycle progression is a major source of variability in live-cell phenotyping, yet imaging workflows still lack a general way to account for it alongside structural and functional readouts. Here we show CALIPERS (Cell-cycle-Aware Live-cell Imaging for Phenotyping Experiments and Regeneration Studies), an integrated framework that pairs a spectrally redesigned FUCCI reporter with continuous cell-cycle inference and flexible delivery strategies in human cells and stem-cell models. In epithelial cells, CALIPERS simultaneously images actin, tubulin, or calcium dynamics, phase-locks migration and proliferation, triggers mitosis-aware smart microscopy, and recovers fast calcium signals. In induced pluripotent stem-cell workflows, lentiviral and safe-harbor versions span pluripotent, lineage-restricted, and cardiac models. In cardiac organoids, CALIPERS tracks cell-cycle exit, tissue compaction, and calcium onset, and distinguishes productive proliferation from multinucleation and endoreplication. By making cell-cycle state an explicit, measurable variable, CALIPERS helps triage candidate regenerative interventions across diverse live-imaging assays.