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<p>H3K27ac and H3K4me3 are enriched at transcriptional start sites and have been implicated in transcription. However, how these marks concertedly regulate transcription is not fully understood. Here, we develop a dual chemically inducible CRISPR/dCas9-based epigenome editing system that enables independent, temporal and transcription stage-specific modulation of H3K27ac and H3K4me3 at a specific gene locus. Stage-specific removal of H3K4me3 impairs RNA polymerase II recruitment, increases promoter-proximal pausing, reduces productive elongation, and accelerates mRNA decay via increased m⁶A deposition. Losing both H3K27ac and H3K4me3 rapidly abolishes transcriptional activity, while preserving H3K4me3 without H3K27ac can partially sustain transcription. These findings reveal a functional hierarchy and interdependence between H3K27ac and H3K4me3 in different transcription stages at the tested gene loci. This versatile tool will contribute to the functional dissection of the temporal dynamics of chromatin modifications in gene regulation.</p>

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Hierarchical interplay between H3K27ac and H3K4me3 in transcriptional regulation

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年6月22日

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  • Chenwei Zhou,
  • Chanjuan Dong,
  • Weiye Zhao,
  • Fu-Sen Liang

摘要

H3K27ac and H3K4me3 are enriched at transcriptional start sites and have been implicated in transcription. However, how these marks concertedly regulate transcription is not fully understood. Here, we develop a dual chemically inducible CRISPR/dCas9-based epigenome editing system that enables independent, temporal and transcription stage-specific modulation of H3K27ac and H3K4me3 at a specific gene locus. Stage-specific removal of H3K4me3 impairs RNA polymerase II recruitment, increases promoter-proximal pausing, reduces productive elongation, and accelerates mRNA decay via increased m⁶A deposition. Losing both H3K27ac and H3K4me3 rapidly abolishes transcriptional activity, while preserving H3K4me3 without H3K27ac can partially sustain transcription. These findings reveal a functional hierarchy and interdependence between H3K27ac and H3K4me3 in different transcription stages at the tested gene loci. This versatile tool will contribute to the functional dissection of the temporal dynamics of chromatin modifications in gene regulation.