Scholarlink

<p>Rice root-knot nematode (RRKN, <i>Meloidogyne graminicola</i>) is a destructive nematodes threatening global rice production. Few RRKN-resistant resources have been reported and their natural variations remain largely unknown. Here, we identify <i>RRKN susceptibility factor 1</i> (<i>Rsf1</i>), encoding a cysteine-rich protein, via Genome-Wide Association Study (GWAS), multi-omics analysis, and functional validation. A natural variation in the TATA-box of <i>OsRsf1</i> is observed, wherein RRKN-susceptible rice accessions contain haplotype (Hap) A while resistant accessions contain HapG. <i>OsRsf1</i> with HapA is highly expressed and induced by RRKN infection, while HapG <i>OsRsf1</i> shows low expression, conferring enhanced RRKN resistance. We further demonstrate that transcription factor Myb44 promotes <i>OsRsf1</i> expression. Knockouts of <i>OsRsf1</i> in rice and its ortholog <i>AtRsf1</i> in Arabidopsis significantly enhanced nematode resistance, indicating that functional conservation. Mutations in <i>OsRsf1</i> trigger a general defence response during RRKN infection. Our finding reveals a conserved <i>Rsf1</i>-mediated susceptibility mechanism underlying RRKN infection and provides a promising target for breeding nematode-resistant crops.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A natural variation in rice susceptibility factor Rsf1 confers resistance to root-knot nematode

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年6月22日

查看全文
  • Jingwen Yu,
  • Chao Xiang,
  • Deliang Peng,
  • Huan Peng,
  • Shiming Liu,
  • Lingan Kong,
  • Ling Qing,
  • Mingjun Li,
  • Xiyue Yu,
  • Huanhuan Hao,
  • Yaqin Liu,
  • Wencui Wu,
  • Xue Han,
  • Yanxue Chen,
  • Houxiang Kang,
  • Wenkun Huang

摘要

Rice root-knot nematode (RRKN, Meloidogyne graminicola) is a destructive nematodes threatening global rice production. Few RRKN-resistant resources have been reported and their natural variations remain largely unknown. Here, we identify RRKN susceptibility factor 1 (Rsf1), encoding a cysteine-rich protein, via Genome-Wide Association Study (GWAS), multi-omics analysis, and functional validation. A natural variation in the TATA-box of OsRsf1 is observed, wherein RRKN-susceptible rice accessions contain haplotype (Hap) A while resistant accessions contain HapG. OsRsf1 with HapA is highly expressed and induced by RRKN infection, while HapG OsRsf1 shows low expression, conferring enhanced RRKN resistance. We further demonstrate that transcription factor Myb44 promotes OsRsf1 expression. Knockouts of OsRsf1 in rice and its ortholog AtRsf1 in Arabidopsis significantly enhanced nematode resistance, indicating that functional conservation. Mutations in OsRsf1 trigger a general defence response during RRKN infection. Our finding reveals a conserved Rsf1-mediated susceptibility mechanism underlying RRKN infection and provides a promising target for breeding nematode-resistant crops.