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<p>Genetically encoded calcium indicators (GECIs) are vital tools for fluorescence-based visualization of neuronal activity with high spatial and temporal resolution. However, current highest-performance GECIs are predominantly green or red fluorescent, limiting multiplexing options and efficient excitation with fixed-wavelength femtosecond lasers operating at 1030 nm. In an effort to overcome these limitations, we developed OCaMP, an orange fluorescent GECI engineered from O-GECO1 through targeted substitutions to improve calcium affinity while retaining the favorable photophysical properties of mOrange2. OCaMP exhibits improved two-photon cross-section, responsiveness, photostability, and calcium affinity relative to O-GECO1. In cultured neurons, zebrafish, and mouse cortex, OCaMP outperforms the red GECIs jRCaMP1a and jRGECO1a in sensitivity, photostability, and signal-to-noise ratio. Here we show that OCaMP, an orange fluorescent GECI, is a robust tool for high-fidelity neural imaging optimized for wavelengths above 1000 nm and a practical option within the spectral gap between existing green and red GECIs.</p>

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A sensitive orange fluorescent calcium ion indicator for imaging neural activity

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年6月22日

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  • Abhi Aggarwal,
  • Heather A. Baker,
  • Céline D. Dürst,
  • I-Wen Chen,
  • Pablo de Chambrier,
  • Carrie Stine,
  • Julia Marie Gonzales,
  • Jonathan S. Marvin,
  • Milène Vandal,
  • Torgny Lundberg,
  • Cherie A. Brown-Panton,
  • Kenryo Sakoi,
  • Ronak Patel,
  • Ching-Yao Wang,
  • Frank Visser,
  • Yannick Fouad,
  • Smrithi Sunil,
  • Matthew Wiens,
  • Takuya Terai,
  • Kei Takahashi-Yamashiro,
  • Roger J. Thompson,
  • Timothy A. Brown,
  • Yusuke Nasu,
  • Minh Dang Nguyen,
  • Grant R. J. Gordon,
  • Sarah McFarlane,
  • Kaspar Podgorski,
  • Anthony Holtmaat,
  • Robert E. Campbell,
  • Alexander W. Lohman

摘要

Genetically encoded calcium indicators (GECIs) are vital tools for fluorescence-based visualization of neuronal activity with high spatial and temporal resolution. However, current highest-performance GECIs are predominantly green or red fluorescent, limiting multiplexing options and efficient excitation with fixed-wavelength femtosecond lasers operating at 1030 nm. In an effort to overcome these limitations, we developed OCaMP, an orange fluorescent GECI engineered from O-GECO1 through targeted substitutions to improve calcium affinity while retaining the favorable photophysical properties of mOrange2. OCaMP exhibits improved two-photon cross-section, responsiveness, photostability, and calcium affinity relative to O-GECO1. In cultured neurons, zebrafish, and mouse cortex, OCaMP outperforms the red GECIs jRCaMP1a and jRGECO1a in sensitivity, photostability, and signal-to-noise ratio. Here we show that OCaMP, an orange fluorescent GECI, is a robust tool for high-fidelity neural imaging optimized for wavelengths above 1000 nm and a practical option within the spectral gap between existing green and red GECIs.