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<p>Chromatin immunoprecipitation (ChIP) has been a cornerstone for epigenetic analyses over the last decades, but even coupled to sequencing approaches (ChIP-seq), it is ultimately limited to one protein at a time. In a complementary effort, we here combine ChIP with label-free quantitative mass spectrometry (qChIP-MS) to interrogate local chromatin compositions. We demonstrate the versatility of our approach at telomeres, with transcription factors, in tissue and by dCas9-mediated locus-specific enrichment.</p>

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qChIP-MS reveals the local chromatin composition by label-free quantitative proteomics

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月26日

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  • Wai Khang Yong,
  • Nurkaiyisah Zaal Anuar,
  • Zi Wayne Sin,
  • Elayne Su Ru Tan,
  • Grishma Rane,
  • Xiaoman Shao,
  • Chai Yeen Goh,
  • Vartika Khanchandani,
  • Vivian L. S. Kuan,
  • Peh Fern Ong,
  • Maya Jeitany,
  • H. Phillip Koeffler,
  • Lih-Wen Deng,
  • Oliver Dreesen,
  • Takaomi Sanda,
  • Dennis Kappei

摘要

Chromatin immunoprecipitation (ChIP) has been a cornerstone for epigenetic analyses over the last decades, but even coupled to sequencing approaches (ChIP-seq), it is ultimately limited to one protein at a time. In a complementary effort, we here combine ChIP with label-free quantitative mass spectrometry (qChIP-MS) to interrogate local chromatin compositions. We demonstrate the versatility of our approach at telomeres, with transcription factors, in tissue and by dCas9-mediated locus-specific enrichment.