Scholarlink

<p>Engineering functional soft tissue constructs remains difficult because scaffolds must meet the mechanical, physicochemical, and biological requirements simultaneously. Here, we present a cell-laden hydrogel gyroid scaffold designed to support vascularisation, long-term multicellular culture, and implantation for the development of 3D tissue constructs. The scaffold is structurally optimised to balance nutrient transport and mechanical stability, and is fabricated with high fidelity by mitigating cell-induced light scattering. The gyroid architecture supports the formation of dense microvascular networks throughout the 3D construct, and its curved Gaussian curvature promotes endothelial self-assembly. The scaffold also enables high-density co-culture of HepG2 cells and HUVECs without active perfusion, resulting in a vascularised 3D liver tumour model with enhanced tissue-specific function. Upon subcutaneous implantation in mice, the constructs show enhanced neovascularisation and facilitate tumour formation. These findings identify the gyroid scaffold as a biologically favourable architecture for generating bulk vascularised constructs, with potential applications in disease modelling, drug screening and regenerative medicine.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

3D triply periodic minimal surface gyroid hydrogel scaffolds for soft tissue engineering

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年5月19日

查看全文
  • Xiaoxiao Han,
  • Ning He,
  • Dandan Yuan,
  • Na Li,
  • Wei Zhu,
  • Xiaolong Zhu,
  • Jing Li,
  • Xun Yuan,
  • Wenxin Wang,
  • Xingshi Gu,
  • Feng Chen,
  • Wei Wang

摘要

Engineering functional soft tissue constructs remains difficult because scaffolds must meet the mechanical, physicochemical, and biological requirements simultaneously. Here, we present a cell-laden hydrogel gyroid scaffold designed to support vascularisation, long-term multicellular culture, and implantation for the development of 3D tissue constructs. The scaffold is structurally optimised to balance nutrient transport and mechanical stability, and is fabricated with high fidelity by mitigating cell-induced light scattering. The gyroid architecture supports the formation of dense microvascular networks throughout the 3D construct, and its curved Gaussian curvature promotes endothelial self-assembly. The scaffold also enables high-density co-culture of HepG2 cells and HUVECs without active perfusion, resulting in a vascularised 3D liver tumour model with enhanced tissue-specific function. Upon subcutaneous implantation in mice, the constructs show enhanced neovascularisation and facilitate tumour formation. These findings identify the gyroid scaffold as a biologically favourable architecture for generating bulk vascularised constructs, with potential applications in disease modelling, drug screening and regenerative medicine.