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<p>Translational control is vital during the maternal-to-zygotic transition (MZT), yet the landscape of embryonic transfer RNA (tRNA) pools has been difficult to explore. Here, we develop Optimized Reaction for Accurate Capture of Low-input Entities tRNA Sequencing (ORACLE-tRNAseq), enabling robust tRNA profiling from as few as five mouse oocytes. We map tRNA landscapes from oocyte to blastocyst, identifying a distinct transition to embryonic tRNA repertoires and upregulation of tRNA pseudogenes at the 4-cell stage. Integrated multi-omics analyses reveal that zygotic tRNA gene activation coincides with zygotic genome activation (ZGA) and correlates with H3K4me3 establishment and chromatin remodeling. By coupling ORACLE-tRNAseq with Ribo-seq, we demonstrate that embryonic tRNA anticodon pools coordinate with high translation-efficiency gene pools to preferentially establish the zygotic translation machinery, particularly from major ZGA onwards. Collectively, these findings provide a resource for understanding translational regulatory networks during early embryogenesis.</p>

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Deciphering tRNA repertoires and translation coordination during mouse early embryogenesis by ORACLE-tRNAseq

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年4月30日

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  • Yun Li,
  • Xin Wang,
  • Ruofan Huang,
  • Haoquan Yu,
  • You Wu,
  • Ze Shu,
  • Wenlin Jiang,
  • Zheng Cao,
  • Zongyu Yu,
  • Hongyan Li,
  • Rushuang Yan,
  • Yawei Gao,
  • Xiao-Ou Zhang,
  • Yunfang Zhang

摘要

Translational control is vital during the maternal-to-zygotic transition (MZT), yet the landscape of embryonic transfer RNA (tRNA) pools has been difficult to explore. Here, we develop Optimized Reaction for Accurate Capture of Low-input Entities tRNA Sequencing (ORACLE-tRNAseq), enabling robust tRNA profiling from as few as five mouse oocytes. We map tRNA landscapes from oocyte to blastocyst, identifying a distinct transition to embryonic tRNA repertoires and upregulation of tRNA pseudogenes at the 4-cell stage. Integrated multi-omics analyses reveal that zygotic tRNA gene activation coincides with zygotic genome activation (ZGA) and correlates with H3K4me3 establishment and chromatin remodeling. By coupling ORACLE-tRNAseq with Ribo-seq, we demonstrate that embryonic tRNA anticodon pools coordinate with high translation-efficiency gene pools to preferentially establish the zygotic translation machinery, particularly from major ZGA onwards. Collectively, these findings provide a resource for understanding translational regulatory networks during early embryogenesis.