Scholarlink

<p>Cell-free systems have great potential for nucleic acid assays, yet universal onsite diagnostics without preamplification are limited. Here, a universal cell-free diagnostic platform termed TRACKer (Target-Responsive non-preAmplification Cell-free diagnostic Kit) has been developed for detecting multiple respiratory viral RNAs in laboratory and field settings. TRACKer integrates three modules: ribozyme allostery module, riboregulator activation module, and output module. The ribozyme allostery module enables universal target recognition through strand displacement-mediated ribozyme conformational switching. The riboregulator activation module achieves preamplification-free detection via cascade expression of reporter proteins. The output module provides swappable reporter templates for luminescent quantification and lateral flow visualization in diverse scenarios. TRACKer is a robust system that enables rapid ( &lt; 70 min) nucleic acid detection without preamplification and demonstrates attomolar sensitivity (1-10 aM) for six respiratory viruses. Overall, TRACKer presents a promising approach to nucleic acid detection, offering a low-cost and scalable solution with potential applications in point-of-care diagnostics and beyond.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

De novo-designed ribozyme-controlled riboregulator for cell-free diagnostics

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月8日

查看全文
  • Yu Tang,
  • Jie Luo,
  • Ben Niu,
  • Binbin Xie,
  • Yaling Yuan,
  • Hongzhao Yang,
  • Shuang Zhao,
  • Ping Huang,
  • Zuowei Xie,
  • Jing Sheng,
  • Ruijia Deng,
  • Jingsen Cao,
  • Jiaqi Liu,
  • Meilin Gong,
  • Shuang Xie,
  • Ming Chen,
  • Kai Chang

摘要

Cell-free systems have great potential for nucleic acid assays, yet universal onsite diagnostics without preamplification are limited. Here, a universal cell-free diagnostic platform termed TRACKer (Target-Responsive non-preAmplification Cell-free diagnostic Kit) has been developed for detecting multiple respiratory viral RNAs in laboratory and field settings. TRACKer integrates three modules: ribozyme allostery module, riboregulator activation module, and output module. The ribozyme allostery module enables universal target recognition through strand displacement-mediated ribozyme conformational switching. The riboregulator activation module achieves preamplification-free detection via cascade expression of reporter proteins. The output module provides swappable reporter templates for luminescent quantification and lateral flow visualization in diverse scenarios. TRACKer is a robust system that enables rapid ( < 70 min) nucleic acid detection without preamplification and demonstrates attomolar sensitivity (1-10 aM) for six respiratory viruses. Overall, TRACKer presents a promising approach to nucleic acid detection, offering a low-cost and scalable solution with potential applications in point-of-care diagnostics and beyond.