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<p>Weak seed dormancy (SD) is prone to pre-harvest sprouting (PHS), which reduces cereal yield and quality. Here, through map-based analysis, we identify <i>TaCNGC-2A</i>, encoding a cyclic nucleotide-gated channel protein, as a negative regulator of wheat SD. Knocking out of <i>TaCNGC-2A</i> enhances SD and PHS resistance, with no yield penalty. Two transcription factors, TaMYB-5B and TaMYB-5D, directly bind to the T/A mutation site of <i>TaCNGC-2A</i> promoter to synergistically repress its expression. The calmodulin TaCaM-3A interacts with TaCNGC-2A to jointly modulate SD and PHS resistance through influencing calcium and multiple hormonal signaling pathways. Knocking out of <i>TaCaM-3A</i> not only enhances SD and PHS resistance, but also increases grain weight and per-plant yield. Finally, we identify allele combinations of <i>TaCNGC-2A</i> and other known dormancy genes associated with strong SD. This study uncovers a regulatory mechanism underlying SD and PHS resistance and provides gene targets for breeding wheat varieties with PHS resistance.</p>

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TaCNGC-2A suppresses seed dormancy and activates pre-harvest sprouting through modulating calcium and hormonal signaling pathways

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年3月27日

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  • Bingbing Tian,
  • Yuhui Fang,
  • Yingjun Zhang,
  • Xinran Cheng,
  • Jiajia Cao,
  • Cheng Kou,
  • Wei Liu,
  • Zhaoyu Yu,
  • Jing Chen,
  • Buyun Li,
  • Huanfeng Wang,
  • Shuying Lei,
  • Wei Gao,
  • Litian Zhang,
  • Yuxia Lv,
  • Shengxing Wang,
  • Hongqi Si,
  • Jie Lu,
  • Can Chen,
  • Wenyang Ge,
  • Cheng Chang,
  • Chuanxi Ma,
  • Yong-Ling Ruan,
  • Haiping Zhang

摘要

Weak seed dormancy (SD) is prone to pre-harvest sprouting (PHS), which reduces cereal yield and quality. Here, through map-based analysis, we identify TaCNGC-2A, encoding a cyclic nucleotide-gated channel protein, as a negative regulator of wheat SD. Knocking out of TaCNGC-2A enhances SD and PHS resistance, with no yield penalty. Two transcription factors, TaMYB-5B and TaMYB-5D, directly bind to the T/A mutation site of TaCNGC-2A promoter to synergistically repress its expression. The calmodulin TaCaM-3A interacts with TaCNGC-2A to jointly modulate SD and PHS resistance through influencing calcium and multiple hormonal signaling pathways. Knocking out of TaCaM-3A not only enhances SD and PHS resistance, but also increases grain weight and per-plant yield. Finally, we identify allele combinations of TaCNGC-2A and other known dormancy genes associated with strong SD. This study uncovers a regulatory mechanism underlying SD and PHS resistance and provides gene targets for breeding wheat varieties with PHS resistance.