Scholarlink

<p>Chloroplast group IIA introns originate from bacterial ribozymes. Their splicing requires the splicing factor Maturase K (MatK). MatK, however, has been difficult to functionally analyze, as it appears essential for plant viability and is encoded in the chloroplast genome. Here we identified a heteromultimeric complex comprising MatK and three other essential, plastid-targeted proteins using co-immunoprecipitation experiments in Arabidopsis and tobacco. Among the MatK interactors is a conserved homologue of starch-branching enzymes (BEs), which we named MATURASE K INTERACTING PROTEIN1 (MKIP1). We demonstrate that MKIP1 proteins have lost BE activity and acquired a 150-amino acid insertion that enables direct interaction with MatK’s N-terminus. Immunoprecipitation of Arabidopsis MKIP1 co-precipitates all known MatK intron targets. Inducing <i>MKIP1</i> silencing in Arabidopsis causes newly emerging leaves to be pale, in which the splicing of MatK intron targets is strongly reduced. Our data suggest that MKIP1 functionally diverged from canonical BEs to facilitate splicing in conjunction with MatK. In turn, the former reverse-transcriptase domain in the N-terminal region of MatK likely has acquired the capacity to interact with other proteins. Potentially, complex formation allowed MatK to diversify its RNA interactions, helping its transition towards a general splicing factor.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Maturase K forms a plastidial splicing complex with a neofunctionalized branching enzyme

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年3月23日

查看全文
  • Yuanyuan Liang,
  • Yang Gao,
  • Andrea Fontana,
  • Melanie Abt,
  • Adam Gicgier,
  • Muriel Gehring,
  • Chun Liu,
  • Mayank Sharma,
  • Reimo Zoschke,
  • Samuel C. Zeeman,
  • Barbara Pfister

摘要

Chloroplast group IIA introns originate from bacterial ribozymes. Their splicing requires the splicing factor Maturase K (MatK). MatK, however, has been difficult to functionally analyze, as it appears essential for plant viability and is encoded in the chloroplast genome. Here we identified a heteromultimeric complex comprising MatK and three other essential, plastid-targeted proteins using co-immunoprecipitation experiments in Arabidopsis and tobacco. Among the MatK interactors is a conserved homologue of starch-branching enzymes (BEs), which we named MATURASE K INTERACTING PROTEIN1 (MKIP1). We demonstrate that MKIP1 proteins have lost BE activity and acquired a 150-amino acid insertion that enables direct interaction with MatK’s N-terminus. Immunoprecipitation of Arabidopsis MKIP1 co-precipitates all known MatK intron targets. Inducing MKIP1 silencing in Arabidopsis causes newly emerging leaves to be pale, in which the splicing of MatK intron targets is strongly reduced. Our data suggest that MKIP1 functionally diverged from canonical BEs to facilitate splicing in conjunction with MatK. In turn, the former reverse-transcriptase domain in the N-terminal region of MatK likely has acquired the capacity to interact with other proteins. Potentially, complex formation allowed MatK to diversify its RNA interactions, helping its transition towards a general splicing factor.