Scholarlink

<p>Succinic acid is an important platform chemical traditionally produced via energy-intensive and environmentally unfriendly processes. <i>Actinobacillus succinogenes</i> offers a sustainable biosynthetic route, yet its productivity is constrained by limited intracellular electron transfer. Here, we develop a photoelectrocatalytic–microbial biohybrid system to overcome these metabolic bottlenecks. Adaptive laboratory evolution using gold nanoparticles establishes an enhanced charge-transfer pathway in <i>Actinobacillus succinogenes</i>, which is subsequently immobilized on a layer-by-layer NiO@PAA@NHS (NiO nanosheets coated with hydrogel of poly acrylic acid (PAA) grafted with N-Hydroxysuccinimide (NHS)) photoelectrode to construct a NiO@PAA@NHS/Au@ <i>Actinobacillus succinogenes</i> biohybrid. Under simulated solar illumination at −0.3 V vs. RHE, the system delivers a photocurrent density of 1.9 mA cm<sup>-2</sup>, a CO<sub>2</sub> conversion efficiency of 67%, and a succinic acid production rate of 1.41 ± 0.04 g L<sup>-1</sup> h<sup>-1</sup> cm<sup>-2</sup>. This work demonstrates an effective strategy for coupling solar energy with microbial metabolism for scalable, carbon-neutral chemical production.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Photoelectrocatalytic-microbial biohybrid for succinic acid synthesis

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年2月24日

查看全文
  • Tianhang Feng,
  • Xue Zhou,
  • Yingjie Zhang,
  • Zhonghai Zhang

摘要

Succinic acid is an important platform chemical traditionally produced via energy-intensive and environmentally unfriendly processes. Actinobacillus succinogenes offers a sustainable biosynthetic route, yet its productivity is constrained by limited intracellular electron transfer. Here, we develop a photoelectrocatalytic–microbial biohybrid system to overcome these metabolic bottlenecks. Adaptive laboratory evolution using gold nanoparticles establishes an enhanced charge-transfer pathway in Actinobacillus succinogenes, which is subsequently immobilized on a layer-by-layer NiO@PAA@NHS (NiO nanosheets coated with hydrogel of poly acrylic acid (PAA) grafted with N-Hydroxysuccinimide (NHS)) photoelectrode to construct a NiO@PAA@NHS/Au@ Actinobacillus succinogenes biohybrid. Under simulated solar illumination at −0.3 V vs. RHE, the system delivers a photocurrent density of 1.9 mA cm-2, a CO2 conversion efficiency of 67%, and a succinic acid production rate of 1.41 ± 0.04 g L-1 h-1 cm-2. This work demonstrates an effective strategy for coupling solar energy with microbial metabolism for scalable, carbon-neutral chemical production.