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<p>Photosynthesis genes in plant chloroplasts are transcribed by the plastid-encoded RNA polymerase called PEP. Consequently, PEP-deficient mutants cannot generate a photosynthetic apparatus and develop non-viable albino seedlings. Inducible complementation of such mutants thus could provide interesting insights in PEP action and chloroplast biogenesis. Here we show the effects of photo-inducible complementation in the albino <i>Arabidopsis</i> mutant <i>pap7-1</i> using a red/blue optoswitch with monochromatic LEDs. Expression of a blue-light-induced <i>PAP7</i> construct that is silent under red light reconstitutes PEP at any time point of <i>pap7-1</i> development resulting in proper chloroplast biogenesis that rescues the non-viable mutant. Induction of chloroplast biogenesis, however, can only occur in very young leaf tissues indicating the existence of a cell-autonomous, biogenic coupling between cell and organelle development. We further uncover that initial PEP formation and function is independent of photosynthesis. The optoswitch termed <i>blue-light valved biogenesis</i> opens experimental avenues to study non-viable plant mutants.</p>

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A red/blue optoswitch for temporal control of chloroplast transcription and biogenesis in Arabidopsis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月20日

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  • Finia Uecker,
  • Frederik M. Ahrens,
  • Tim Ruder,
  • Thomas Pfannschmidt

摘要

Photosynthesis genes in plant chloroplasts are transcribed by the plastid-encoded RNA polymerase called PEP. Consequently, PEP-deficient mutants cannot generate a photosynthetic apparatus and develop non-viable albino seedlings. Inducible complementation of such mutants thus could provide interesting insights in PEP action and chloroplast biogenesis. Here we show the effects of photo-inducible complementation in the albino Arabidopsis mutant pap7-1 using a red/blue optoswitch with monochromatic LEDs. Expression of a blue-light-induced PAP7 construct that is silent under red light reconstitutes PEP at any time point of pap7-1 development resulting in proper chloroplast biogenesis that rescues the non-viable mutant. Induction of chloroplast biogenesis, however, can only occur in very young leaf tissues indicating the existence of a cell-autonomous, biogenic coupling between cell and organelle development. We further uncover that initial PEP formation and function is independent of photosynthesis. The optoswitch termed blue-light valved biogenesis opens experimental avenues to study non-viable plant mutants.