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<p>Protein chips and arrays in parallel enable high-throughput protein detection in small-volume biological fluids, however, the limitations in sensitivity, dependence on expensive/bulky external equipment, and the need for specialized technical personnel have restricted their application in clinical and laboratory settings. Here, we present a droplet-based do-it-yourself (DBDIY) array on patterned plasmonic chips for high-throughput immunoassays, via confined droplets and plasmonic enhanced near-infrared (NIR) fluorescence. The assay allows equipment-free microarray fabrication and highly sensitive detection using only a pipette. It dynamically profiles 10 cytokines over 40 days in individual mice with just 6 µL of serum/blood per time point and achieves 100% sensitivity and specificity for C-reactive protein (CRP) in 1&#xa0;nL of peripheral whole blood samples from 112 children. Scalable to high-throughput analysis of cell culture and serum, this accessible system simplifies workflows, extends utility in resource-limited settings, and offers a next-generation tool for longitudinal animal studies and clinical diagnostics.</p>

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Do-it-yourself protein arrays as high-throughput immunoassays enabled by confined droplets on patterned plasmonic chips

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月14日

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  • Ying Yue,
  • Chenyu Shi,
  • Yichen Wang,
  • Shengjie Ma,
  • Bin Sun,
  • Qiqi Kan,
  • Zhengchao Ji,
  • Shuai Dong,
  • Xue Zheng,
  • Hongli Shan,
  • Zheng Hu,
  • Jiancheng Xu,
  • Shoujun Zhu,
  • Junhu Zhang,
  • Bai Yang

摘要

Protein chips and arrays in parallel enable high-throughput protein detection in small-volume biological fluids, however, the limitations in sensitivity, dependence on expensive/bulky external equipment, and the need for specialized technical personnel have restricted their application in clinical and laboratory settings. Here, we present a droplet-based do-it-yourself (DBDIY) array on patterned plasmonic chips for high-throughput immunoassays, via confined droplets and plasmonic enhanced near-infrared (NIR) fluorescence. The assay allows equipment-free microarray fabrication and highly sensitive detection using only a pipette. It dynamically profiles 10 cytokines over 40 days in individual mice with just 6 µL of serum/blood per time point and achieves 100% sensitivity and specificity for C-reactive protein (CRP) in 1 nL of peripheral whole blood samples from 112 children. Scalable to high-throughput analysis of cell culture and serum, this accessible system simplifies workflows, extends utility in resource-limited settings, and offers a next-generation tool for longitudinal animal studies and clinical diagnostics.