Scholarlink

<p>Viral diseases critically impact crop yields, necessitating insights into plant-pathogen recognition and resistance. In tomato (<i>Solanum lycopersicum</i>), tomato chlorosis virus&#xa0;(ToCV) employs 59 kDa protein (p59) to facilitate movement and degrade the host Catalase1 (SlCAT1), disrupting redox homeostasis. Importantly, tomato has evolved an antiviral ubiquitin-proteasome system (UPS) featuring the <Emphasis Type="Underline">A</Emphasis>nti<Emphasis Type="Underline">v</Emphasis>iral <Emphasis Type="Underline">E2</Emphasis> ubiquitin-conjugating enzyme (SlAVE2) and <Emphasis Type="Underline">A</Emphasis>nti<Emphasis Type="Underline">v</Emphasis>iral <Emphasis Type="Underline">E3</Emphasis> ubiquitin ligase (SlAVE3) pair, which specifically recognizes p59 to trigger antiviral defenses. During infection, this system ubiquitinates SlWRKY6, alleviating its repression of <i>SlAVE3</i> and creating a positive feedback loop that enhances <i>SlAVE3</i> expression. SlAVE3 then targets p59 for ubiquitination and degradation, inhibiting viral spread. Notably, p59 co-opts this UPS to degrade SlCAT1, illustrating a counter-defense interplay. Domestication analysis reveals selection of <i>AVE3</i> from <i>S. pimpinellifolium</i> to <i>S. lycopersicum</i>, with SpAVE3 conferring stronger ToCV resistance than SlAVE3 through improved p59 targeting and reduced SlCAT1 degradation. This study unveils a domesticated antiviral UPS, providing a foundation for breeding ToCV-resistant tomatoes.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Tomato antiviral ubiquitin-proteasome system recognizes viral 59 kDa protein to confer tomato chlorosis virus resistance

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月31日

查看全文
  • Dan Zhao,
  • Xiaoqian Liu,
  • Hongbo Li,
  • Tianchen Xia,
  • Qi Jia,
  • Peidong Li,
  • Qing Shan,
  • Changqian Li,
  • Hui Li,
  • Yilei Gai,
  • Tao Lin,
  • Lianyi Zang,
  • Tao Zhou,
  • Jin-Wei Wei,
  • Xiaoping Zhu,
  • Biao Gong

摘要

Viral diseases critically impact crop yields, necessitating insights into plant-pathogen recognition and resistance. In tomato (Solanum lycopersicum), tomato chlorosis virus (ToCV) employs 59 kDa protein (p59) to facilitate movement and degrade the host Catalase1 (SlCAT1), disrupting redox homeostasis. Importantly, tomato has evolved an antiviral ubiquitin-proteasome system (UPS) featuring the Antiviral E2 ubiquitin-conjugating enzyme (SlAVE2) and Antiviral E3 ubiquitin ligase (SlAVE3) pair, which specifically recognizes p59 to trigger antiviral defenses. During infection, this system ubiquitinates SlWRKY6, alleviating its repression of SlAVE3 and creating a positive feedback loop that enhances SlAVE3 expression. SlAVE3 then targets p59 for ubiquitination and degradation, inhibiting viral spread. Notably, p59 co-opts this UPS to degrade SlCAT1, illustrating a counter-defense interplay. Domestication analysis reveals selection of AVE3 from S. pimpinellifolium to S. lycopersicum, with SpAVE3 conferring stronger ToCV resistance than SlAVE3 through improved p59 targeting and reduced SlCAT1 degradation. This study unveils a domesticated antiviral UPS, providing a foundation for breeding ToCV-resistant tomatoes.