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<p>Rice paddies significantly contribute to atmospheric methane (CH<sub>4</sub>). Here, we show that two independent rice genotypes overexpressing genes for <i>PLANT PEPTIDES CONTAINING SULFATED TYROSINE</i> (<i>PSY</i>) reduce cumulative CH<sub>4</sub> emissions by 38% (PSY1) and 58% (PSY2) over 70 days of growth compared with controls. Genome-resolved metatranscriptomic data from PSY rhizosphere soils reveal lower ratios of gene activities for (mostly hydrogenotrophic) CH<sub>4</sub> production versus consumption, decreased activity of H<sub>2</sub>-producing genes, and increased activity of bacterial H<sub>2</sub> oxidation pathways. Metabolic modeling using metagenomic and metabolomic data predicts elevated H<sub>2</sub> oxidation and suppressed H<sub>2</sub> production in the PSY rhizosphere. Assembled genomes of rhizosphere H<sub>2</sub>-oxidizing bacteria are enriched in genes utilizing gluconeogenic acids compared with H<sub>2</sub>-producing counterparts, and their activities are likely stimulated by elevated levels of gluconeogenic acids, primarily amino acids, in PSY root exudates. Overall, our study indicates that decreased CH<sub>4</sub> emissions are due to a lower amount of H<sub>2</sub> available for hydrogenotrophic methanogenesis and provides a powerful strategy to mitigate CH<sub>4</sub> emissions from increasingly widespread rice cultivation.</p>

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Reduced methane emissions in transgenic rice genotypes are associated with altered rhizosphere microbial hydrogen cycling

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年1月26日

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  • Ling-Dong Shi,
  • Maria Florencia Ercoli,
  • Junhyeong Kim,
  • Artur Teixeira de Araujo Junior,
  • Katerina Estera-Molina,
  • Subah Soni,
  • Tracy Satomi Weitz,
  • Alexandra M. Shigenaga,
  • Ilija Dukovski,
  • Rohan Sachdeva,
  • Halbay Turumtay,
  • Katherine B. Louie,
  • Benjamin P. Bowen,
  • Suzanne M. Kosina,
  • Henrik V. Scheller,
  • Jennifer Pett-Ridge,
  • Daniel Segrè,
  • Trent R. Northen,
  • Pamela C. Ronald,
  • Jillian F. Banfield

摘要

Rice paddies significantly contribute to atmospheric methane (CH4). Here, we show that two independent rice genotypes overexpressing genes for PLANT PEPTIDES CONTAINING SULFATED TYROSINE (PSY) reduce cumulative CH4 emissions by 38% (PSY1) and 58% (PSY2) over 70 days of growth compared with controls. Genome-resolved metatranscriptomic data from PSY rhizosphere soils reveal lower ratios of gene activities for (mostly hydrogenotrophic) CH4 production versus consumption, decreased activity of H2-producing genes, and increased activity of bacterial H2 oxidation pathways. Metabolic modeling using metagenomic and metabolomic data predicts elevated H2 oxidation and suppressed H2 production in the PSY rhizosphere. Assembled genomes of rhizosphere H2-oxidizing bacteria are enriched in genes utilizing gluconeogenic acids compared with H2-producing counterparts, and their activities are likely stimulated by elevated levels of gluconeogenic acids, primarily amino acids, in PSY root exudates. Overall, our study indicates that decreased CH4 emissions are due to a lower amount of H2 available for hydrogenotrophic methanogenesis and provides a powerful strategy to mitigate CH4 emissions from increasingly widespread rice cultivation.