Scholarlink

<p>Pneumonic plague remains a high-consequence respiratory disease, highlighting the need for vaccines that induce durable lung-localized immunity. We show that intramuscular prime-boost vaccination with Alum-absorbed OMV<sub>46</sub>-LcrV (OMV<sub>46</sub>-LcrV/Alum) is safe but provides limited long-term protection against respiratory <i>Yersinia pestis</i> challenge in mice. To enhance lung immunity, we deliver an adjuvant-free intranasal LcrV “spike” following IM OMV<sub>46</sub>-LcrV/Alum priming. This “prime-spike” regimen is well tolerated and confers complete short- and long-term protection against high-dose pulmonary <i>Y. pestis</i> challenge. The approach increases lung resident memory B cells, including antibody-secreting cells, and resident memory T cells with elevated Interferon-γ, Interleukin-17A, and Interleukin-4 production. These localized memory responses contribute to improved protection, demonstrating the promising potential of prime-spike immunization for combating pneumonic plague.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Intranasal unadjuvanted LcrV boosts parental Yersinia OMV primed lung immunity against pneumonic plague in mice

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月13日

查看全文
  • Saugata Majumder,
  • Shreya Das,
  • Mohd Saqib,
  • McKenzie Van der Veer,
  • Moe Acee,
  • Wei Sun

摘要

Pneumonic plague remains a high-consequence respiratory disease, highlighting the need for vaccines that induce durable lung-localized immunity. We show that intramuscular prime-boost vaccination with Alum-absorbed OMV46-LcrV (OMV46-LcrV/Alum) is safe but provides limited long-term protection against respiratory Yersinia pestis challenge in mice. To enhance lung immunity, we deliver an adjuvant-free intranasal LcrV “spike” following IM OMV46-LcrV/Alum priming. This “prime-spike” regimen is well tolerated and confers complete short- and long-term protection against high-dose pulmonary Y. pestis challenge. The approach increases lung resident memory B cells, including antibody-secreting cells, and resident memory T cells with elevated Interferon-γ, Interleukin-17A, and Interleukin-4 production. These localized memory responses contribute to improved protection, demonstrating the promising potential of prime-spike immunization for combating pneumonic plague.