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<p>As a sister genus to <i>Taxus</i>, <i>Pseudotaxus</i> holds significant importance for studying the origin and evolution of the taxane biosynthesis pathway. However, the reference genome of <i>Pseudotaxus chienii</i> is yet unavailable. Here, we report a chromosome-level genome assembly of <i>P. chienii</i> (15.6 Gb). We show that <i>P. chienii</i> only possesses a partial taxane pathway, which terminates before taxane 2α-<i>O</i>-benzoyl transferase (TBT), a crucial enzyme responsible for the production of 10-deacetylbaccatin III. With the emergence of the <i>Taxus</i> genus, the limitation posed by the lacking of functional TBT is overcome, allowing for the extension of the existing taxane biosynthesis pathway into a complete Taxol biosynthesis. The protein structure of metal ion catalysis sites in taxadiene synthase (TS) is conserved across the <i>Pseudotaxus</i> and <i>Taxus</i> genera, providing potential sites for enhancing TS activity through enzyme engineering. This comparative genomic analysis contributes to our understanding of the origin and evolution of taxane biosynthesis within the Taxaceae family.</p>

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Analysis genome of Pseudotaxus chienii reveals insights into the origin and evolution of taxane biosynthesis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年12月18日

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  • Mingshuang Wang,
  • Ruoyun Ma,
  • Zijin Fang,
  • Lingxiao Zhang,
  • Yibo Zhang,
  • Mengyin Zheng,
  • Enhui Bai,
  • Wanting Lin,
  • Yanyan Pei,
  • Yue Zang,
  • Huijie Ma,
  • Shangguo Feng,
  • Xiaori Zhan,
  • Chunna Yu,
  • Shuling Wang,
  • Huizhong Wang,
  • Chenliang Yu,
  • Chenjia Shen

摘要

As a sister genus to Taxus, Pseudotaxus holds significant importance for studying the origin and evolution of the taxane biosynthesis pathway. However, the reference genome of Pseudotaxus chienii is yet unavailable. Here, we report a chromosome-level genome assembly of P. chienii (15.6 Gb). We show that P. chienii only possesses a partial taxane pathway, which terminates before taxane 2α-O-benzoyl transferase (TBT), a crucial enzyme responsible for the production of 10-deacetylbaccatin III. With the emergence of the Taxus genus, the limitation posed by the lacking of functional TBT is overcome, allowing for the extension of the existing taxane biosynthesis pathway into a complete Taxol biosynthesis. The protein structure of metal ion catalysis sites in taxadiene synthase (TS) is conserved across the Pseudotaxus and Taxus genera, providing potential sites for enhancing TS activity through enzyme engineering. This comparative genomic analysis contributes to our understanding of the origin and evolution of taxane biosynthesis within the Taxaceae family.